应用通用寡核苷酸芯片技术检测病原菌的研究

Applied Study on Oligonucleotide Microarray Technology to Detect Pathogens

本文以DNA为靶标,针对待检细菌制备寡核苷酸芯片,根据杂交条件进行摸索,然后重新确立寡核苷酸芯片,通过结果的相似性来判断样本的种类。检测中利用通用引物SUA和GO7B对涉及40多个进行扩增和标记后,针对不同的种、属的混合模式,增加它们相互之间的分辨能力,同样样品中存在多个菌种时也可检测出来,并为临床诊断提供依据。综合比较,该项检测技术具有良好的使用价值。

Targeting to DNA, preparation of oligonucleotide microarray for the bacteria to be detected under hybridization conditions were found, and then re-establishment of oligonucleotide microarray, to judge by similarity of the results of sample types. Using universal primers SUA and G07B in the detection after ampliifcation and label involved more than 40 more for different species and genus of blend modes, increase their ability to distinguish between each other, as when there are multiple strains can also be detected in a sample and provide the basis for the clinical diagnosis. The detection technology is a good value.