罗格列酮联合依那普利对腹膜纤维化大鼠的腹膜功能的影响

Impact of Both Rosiglitazone(RGZ) and Enalapril on Peritoneal Function and Cytokines in Peritoneum of Rat Peritoneal Fibrotic Model

目的：研究观察罗格列酮联合依那普利对腹膜透析所致腹膜纤维化的影响，探讨其对腹膜纤维化的作用及可能的机制。方法80只SD大鼠（6～8周龄，体重180～200 g）随机分为4组，每组20只。分为对照组、模型组、依那普利治疗组和罗格列酮联合依那普利联合用药组。除对照组外其余组均采用高糖腹膜透析液＋红霉素制作慢性腹膜透析无菌性腹膜纤维化的大鼠模型。上述各组大鼠均于腹腔注射7周后处死；进行1小时腹膜平衡试验，用免疫组化方法测定各组的FN以及TGF-β1的表达水平。结果罗格列酮联合依那普利治疗组的腹膜超滤功能增加更明显。免疫组化分析，联合治疗组中的FN以及TGF-β1的表达率较模型组减低更显著。结论罗格列酮联合依那普利治疗组可降低转化生长因子的表达，减轻腹膜纤维化起到保护腹膜的作用。

ObjectiveTo observe impact of both rosiglitazone （RGZ） and enalapril on cytokines in peritoneum of rat peritoneal fibrotic model and study effect and mechanism of their effect on peritoneal fibrosis in rats. Methods 80 male Sprague-Dawley rats were randomly divided into 4 groups： control group, model group, enalapril group and both rosiglitazone （RGZ） and enalapril group. Except for normal group , other rats were treatment daily intraperitoneal injection of 4.25% dialysate（75 ml/kg body weight） combined with four times injection of erythromycin lactobionate for 7 weeks; After seven weeks, a one-hour peritoneal equilibration test （PET） was performed to detect peritoneal function; TGF-β and FN levels were determined in immunohistochemical method. Results Both rosiglitazone （RGZ） and enalapril group, peritoneal ultrafiltration function was more increased more signiifcantly in treatment group. The expression ratio of FN and TGF-β1 was more evidently downregulated in treatment group （P〈0.01）. Conclusion Both rosiglitazone （RGZ） and enalapril may suppress express of TGF-β, reduce extracellular matrix FN accumulation, improve peritoneal ifbrosis and protect peritoneum.