UPLC测定冻干培养基中甲氧苄啶的含量

Determination of Freeze-drying Medium UPLC Trimethoprim

目的:建立测定冻干培养基中甲氧苄啶含量的超高效液相色谱(UPLC)法,测定培养基中的甲氧苄啶含量。方法:采用ACQUITY UPLC HSS T3 1.8μm(2.1*100 mm)column为色谱柱,以水∶乙腈∶三乙胺=799∶200∶1(冰醋酸调节pH至5.4)为流动相,检测波长为271 nm,流速为0.2mL/min,进样量:2μL,柱温:30℃。结果:在方法学学考察中,系统适应性试验测定时甲氧苄啶的保留时间为2.782 min,峰形良好,基线平稳,样品中的内源性物质及溶剂等对测定无干扰。精密度试验中RSD=0.27%,说明该方法精密度较好,实验结果准确性可靠。重复性试验中RSD=0.17%,说明在实验过程中操作规范,误差较小。加样回收率:RSD=0.19%说明该方法的精密度好,测定结果可信。甲氧苄啶在冻干培养基中占比为0.00706,即每1 mg混合物中有甲氧苄啶0.00706 mg。结论:操作简单,结果准确,重现性好,可用于甲氧苄啶的含量测定。

Objective： Determination of freeze-dried to build ultra performance liquid chromatography medium trimethoprim content（UPLC） method,determination of medium trimethoprim content.Methods： ACQUITY UPLC HSS T3 1.8 μm（2.1 ＊ 100 mm） column to column with water ∶ acetonitrile ∶triethylamine=799∶200∶1（glacial acetic acid to adjust pH to 5.4） as the mobile phase,detection wavelength of 271 nm,a flow rate of 0.2mL/min,injection volume：2 μL,column temperature： 30 ℃.Results： In the method to learn the inspection,the system suitability test measures trimethoprim retention time 2.782 min,good peak shape,smooth baseline endogenous substances in the sample and solvent on the determination of non-interference.Precision tests RSD=0.27 %,indicating that the precision of the method is better,reliable and accurate results.Repeatability tests RSD=0.17 %,instructions norms,the error is small during the experiment.Recoveries： RSD=0.19 % precision of the method is a good description of the measurement results credible.Trimethoprim in lyophilization medium accounting for0.00706,per 1mg of the mixture with trimethoprim 0.00706 mg.Conclusion： simple,accurate,reproducible,and can be used for content trimethoprim tablets.