Hes1在大鼠脊髓发育不同阶段的表达

Expression of the Hes1 in the development process of rat spinal cord

目的:检测Hes1在大鼠脊髓发育过程中不同阶段的表达。方法:选取胚胎15.5 d、18.5 d和出生当天,出生后3 d、7 d、2周、4周及成年的SD大鼠脊髓,采用Western blot和荧光定量PCR检测Hes1在大鼠脊髓发育过程中的表达。结果:从胚胎15.5 d到成年期,大鼠脊髓中都有Hes1的表达,具有一定的变化规律,总体上来看,胚胎期表达量比较高,出生后的表达降低。结论:Hes1在大鼠脊髓发育过程中的不同阶段表达量有所不同,胚胎期表达量高,出生后表达量明显下降。

Objective： To detect the expression of Hes1 in the development process of rat spinal cord. Methods： The rat spinal cord at embryonic day 15. 5 and 18. 5,the day of birth,3 days after birth,7 days,2 weeks and 4 weeks,and adult rat spinal cord were harvested,the Hes1 expressions of which were detected by the fluorescence quantitative PCR and Western blot. Results： The expressions of Hes1 in the rat spinal cord of 15. 5 d to adulthood were found,which showed certain regular changes. The expressions of Hes1 in embryonic stages of rat was high,which was decreased after birth. Conclusions： The expression of Hes1 at different stages of the rat spinal cord development is different,which was high in the embryonic period,and was depressed after birth.