GSTP1基因变异与胃黏膜非典型增生的关系

The Relationship between GSTP1 Genovariation and Gastric Dysplasia

目的检测胃黏膜非典型增生(GD)及不同程度GD患者谷胱苷肽硫转移酶P1(GSTP1)第5外显子变异情况,探讨GSTP1变异与胃黏膜GD的关系。方法选择2008年3月至2012年12月中国医科大学附属第一医院、沈阳奉天医院以及沈阳第四人民医院收集的150例胃部疾病患者为研究对象,根据疾病类型分为非萎缩性胃炎组(50例)、GD组(50例)以及胃癌组(50例),采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测GSTP1第5外显子变异情况(rs200139798)。结果胃癌组和GD组GSTP1第5外显子携带G等位基因的频率均高于非萎缩性胃炎组(P<0.05);高度GD组携带G等位基因的频率高于非萎缩性胃炎组和低度GD组(P<0.05),与胃癌组相似(P>0.05)。结论 GSTP1第5外显子携带G等位基因的GD患者易发生胃癌,尤其是高度GD患者,临床需予以重视。

Objective To detect the variation status of GSTP1 of exon 5 of the different degrees of gastric dysplasia（ GD） and discuss the relationship between GD and GSTP1 genovariation. Methods Total of150 gastric disease patients from the First Hospital Affiliated to China Medical University,Fengtian Hospital and the Fourth People＇s Hospital of Shenyang during Mar. 2008 and Dec. 2012 were selected. The patients were divided into non-atrophic gastritis group（ 50 cases）,gastric dysplasia group（ 50 cases） and 50 cases of gastric cancer according to the type of diseases. Using polymerase chain reaction restriction fragment length polymorphisms（ PCR-RFLP） method to detect the variation of GSTP1（ rs200139798）. Results Gastric cancer group and GD group carrying G allele frequency of GSTP1 exon 5 were higher than the non-atrophic gastritis group（ P 0. 05）,high degree GD group carrying G allele frequency was higher than non-atrophic gastritis group and low degree GD group（ P 0. 05）,it was similar to the gastric cancer group（ P 0. 05）.Conclusion The GD patients carrying G allele frequency of exon 5 GSTP1 were prone to develop stomach cancer,especially the patients with severe GD,and due attention should be paid in clinical.