摘要
目的:建立食品中罂粟壳成分的快速检测方法,为罂粟壳成分的检测提供依据。方法:对监督执法中抽检的20份样品中的5种罂粟壳成分应用竞争抑制免疫层析快速检测法进行检测,然后利用液相色谱—质谱法对阳性样品进行检测验证。结果:经液相色谱—质谱法和竞争抑制免疫层析法检测,20份样品中有2份检测阳性、18份阴性,合格率90%,竞争抑制免疫层析法阳性检出率100%。结论:竞争抑制免疫层析法快速、便捷,干扰因素少,阳性检出率高,值得应用推广。
【Objective】To establish a rapid method for compenents of poppy shell to provide reliable basis for the detection. 【Method】Totally 20 samples were detected by Competitive Inhibition Assay,and 2 positive samples were validated by Liquid Chromatography-Mass Spectrometry. 【Result】After Liquid Chromatography-Mass Spectrometry and Competitive Inhibition Assay,2 samples were in 20 samples,and the qualified rate was 90%,positive detection rate by the Competitive Inhibition Assay was 100%. 【Conclusion】The Competitive Inhibition Assay was rapid,convenient and less interference,positive detection rate was high,which was worth to be popularized in food supervision.
出处
《中国食物与营养》
2015年第3期8-10,共3页
Food and Nutrition in China
基金
昆山市社会发展科技计划项目(项目编号:KS1355)
关键词
罂粟壳
快速检测方法
食品安全
液相色谱—质谱法
竞争抑制免疫层析法
poppy shell
rapid detection method
food safety
Liquid Chromatography-Mass Spectrometry（LC-MS）
Competitive Inhibition Assay
