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牡丹PseIF5A基因克隆及其初步功能验证 被引量：1

Research on Gene Clone and its Expression,Function in E.coli of Pse IF5A from Peony
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摘要 从热激后耐热的牡丹品种‘凤丹白’中克隆到牡丹Pse IF5A,全长480bp,与葡萄的同源基因具有较高的同源性,为热激诱导表达基因。将该基因转入大肠杆菌后,重组的菌株能诱导表达该基因的融合蛋白,并提高了对高温、低温、重金属、高盐、高Ph、氧化等非生物胁迫的抗性,表明牡丹Pse IF5A能响应多种非生物胁迫。 PseIF5A, a gene induced by heat stress, had been cloned from ‘Fengdanbai＇, one heat-tolerance variety of paeonia suffructicosa and its ORF has 480bp, with hing homology comparison of nucleotide and amino acid sequence in VveIFSA from grape. Recombinant PseIFSA had detected in recombine E.coli BL21 by SDS-PAGE and western blot and its expression had made recombine E. coli increase the abiotic stresses, such as hing temperature, low temperature, heavy metal, salt, hing Ph and oxidation. So, this research result had indicated PseIF5A can response to Various abiotic stresses.
作者 蒋昌华 高燕 叶康 宋壵 张亚利 秦俊 奉树成
机构地区 上海植物园 上海城市植物资源开发应用工程技术研究中心
出处 《现代园艺》 2015年第4期15-17,共3页 contemporary horticulture
基金 上海市绿化和市容管理局2011年科技项目辰山专项<牡丹低温催花技术及其分子机理研究>(JB110325)
关键词 牡丹Pse IF5A基因 克隆 原核表达 非生物胁迫 PseIFSA gene clone E.coli-expression abiotic stress
分类号 S685.11 [农业科学—观赏园艺]
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参考文献25

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  • 4Park M H.The essential role of hypusine in eukaryotic translation initi ation factor 4D(e IF-4D).Purification of e IF-4D and its precursors and comparison of their activities[J].J Biol Chem,1989,264(31).
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二级参考文献29

  • 1姜蕊,胡永红,蒋昌华,赵宏伟,胡尚连,明凤.热胁迫下月季叶片蛋白双向电泳分析[J].中国生物工程杂志,2006,26(4):91-94. 被引量：10
  • 2Gordon E D, Mora R, Meredith S C, et al. Eukaryotic initiation factor 4D, the hypusine-containing protein, is conserved among eukaryotes [J]. J Biol Chem, 1987,262(34) : 16585-16589.
  • 3Park M H, Wolff E C, Folk J E. Hypusine.. Its post-translational formation in eukaryotic initiation factor 5A and its potential role in cellular regulation [J]. Biofactors, 1993,4(2): 95-104.
  • 4Hanauske-Abel H M, Park M H, Hanauske A R, et al. Inhibition of the G1-S transition of the cell cycle by inhibitors of deoxyhypusine hydroxylation [J~. Biochim Biophys Acta, 1994,1221(2) : 115-124.
  • 5Park M H. The essential role of hypusine in eukaryotic translation initiation factor 4D (eIF-4D): Purification of eIF-4D and its precursors and comparison of their activities [J].J Biol Chem, 1989,264 (31) : 18531-18535.
  • 6Kemper W M, Berry K W, Merrick W C. Purification and properties of rabbit reticulocyte protein synthesis initiation factors M2Balpha and M2Bbeta [J]. J Biol Chem, 1976,251(18) : 5551-5557.
  • 7Saini P, Eyler D E, Green R, et al. Hypusine-containing protein elF5A promotes translation elongation [J]. Nature, 2009,459(7243).118-121.
  • 8Gregio A P, Cano V P, Avaca J S, et al. eIF5A has a function in the elongation step of translation in yeast [J].Biochem Biophys Res Commun, 2009,380(4) : 785-790.
  • 9Kang H A, Hershey J W. Effect of initiation factor eIF-5A depletion on protein synthesis and proliferation of Saccharomyces cerevisiae [J].J Biol Chem, 1994,269(6) : 3934-3940.
  • 10Jiao D L, Chen K Y. Subcellular localization of the hypusine-containing eukaryotic initiation factor 5A by immunofluorescent staining and green fluorescent protein tagging[J]. J Cell Biochem, 2002,86(3) : 590- 600.

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现代园艺
2015年 第4期

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