摘要
目的建立四味光明盐汤散中没食子酸的含量测定方法。方法采用高效液相色谱法。色谱柱为Phenomen C18柱（250mm×4.6mm,5μm）;流动相为甲醇-0.1%磷酸（7：93）;检测波长为272nm;流速为1.0ml·min-1;柱温30℃。结果没食子酸在60.00~600.0 ng范围内与峰面积积分值呈良好的线性关系（γ=1.000 0）,回归方程为Y=13 642X＋58 170,平均回收率为99.0%,RSD为1.51%（n=9）。结论该方法准确、精密、重复性好,可作为四味光明盐汤散中没食子酸的含量测定方法。
Objective To establish the determination method of gallic acid in Siwei Guangmingyan Tangsan. Methods Phenomen C18 column（250mm×4.6mm,5μm） was used in HPLC assay; mobile phase was methanol-0.1% phosphoric acid（7：93）; detection wavelength was 272nm; flow rate was 1.0ml·min-1; column temperature was 30℃. Results The calibration curve of gallic acid was linear in the range of 60.00 ng ~600.0ng（γ= 1.000 0）, the regresstion equation was Y=3.281 4X＋24.141, the average recovery rate was 99.0 %, RSD was 1.51%（n=9）. Conclusion The method is accurate, precise and had good reproducibility, which can be used as determination method of gallic acid in Siwei Guangmingyan Tangsan.
出处
《现代中药研究与实践》
CAS
2015年第1期67-68,79,共3页
Research and Practice on Chinese Medicines
基金
内蒙古自治区科技创新引导奖励资金项目(20121502)
