RSK4蛋白在胰腺癌组织中的表达及意义

EXPRESSION AND CLINICOPATHOLOGICAL SIGNIFICANCE OF RSK4 PROTEIN IN PRIMARY PANCREATIC CARCINOMA TISSUES

目的:检测核糖体S6蛋白激酶4(Ribosomal S6protein kinase4,RSK4)在胰腺癌组织中的表达,并探讨其与临床病理参数的关系及预后意义。方法:使用免疫组化检测50例胰腺癌及30例癌旁组织中RSK4蛋白的表达,χ2检验和Spearman相关分析其表达与临床病理参数的关系;Kaplan-Meier单因素分析和COX比例风险模型分析患者预后的影响因素。结果:RSK4蛋白在胰腺癌组织中表达率为28%(14/50),癌旁组织中表达率为50%(15/30),胰腺癌组织表达率明显低于癌旁组织(P<0.05)。RSK4蛋白与血清CA199水平有关(r=-0.288,P<0.05),而与其它临床病理参数无关(P>0.05)。RSK4蛋白与患者术后生存期无关(P>0.05);肿瘤分化程度和TNM临床分期是影响胰腺癌患者预后的因素,且均是独立的预后因素。结论:RSK4蛋白在胰腺癌组织中表达水平降低,提示其可能参与胰腺癌的发生发展。

Objective：To investigate the expression and its clinicopathological and prognostic significance of ribosomal S6 protein kinase4（RSK4）protein in human primary pancreatic carcinoma （PC）.Methods：Immu-nohistochemical （IHC）staining was performed to detect the expression of RSK4 protein in fifty cases of PC and thirty cases of adj acent non-neoplastic pancreatic tissues.The relationship between RSK4 protein level and clinicopathological parameters of PC were analyzed by Chi-square test and Spearman’s rank correla-tion.Kaplan-Meier univariate analysis and multivariate COX proportional hazards models were used to compare prognostic factors.Results：The relative expression level of RSK4 protein in PC was significantly lower than adjacent non-neoplastic pancreatic tissues （P 〈0.05）.The expression of RSK4 protein in PC was reversely related to CA19-9 （r=-0.288,P 〈0.05）,however no statistically significant correlation was found between RSK4 protein expression and other clinicopathological parameters and overall survival （P〉0.05）.The expressions of tumor differentiation and TNM clinical stage were independent prognostic factors of PC.Conclusion：The down-regulation expression of RSK4 protein in PC reminder RSK4 may play a role in the development of PC.