胃腺癌组织中血管活性肠肽及其受体mRNA的表达研究

MRNA EXPRESSION OF VASOACTIVE INTESTINAL PEPTIDE AND ITS RECEPTOR IN GASTRIC ADENOCARCINOMA

目的:研究血管活性肠肽(VIP)及其受体(VIPR)mRNA在胃腺癌组织中的表达情况,探讨其在胃腺癌发病过程中的作用。方法:通过RT-PCR方法,检测32例胃腺癌组织(胃腺癌组)、33例不典型增生胃黏膜组织(不典型增生组)和24例正常胃腺组织(正常对照组)中VIP及VIPR mRNA的表达。结果:3组胃腺组织的VIP mRNA表达率均为100.00%。胃腺癌组的VIP mRNA的相对表达量均高于正常对照组和不典型增生组(P<0.01),正常对照组和不典型增生组之间比较差异无统计学意义(P>0.05)。正常对照组、胃腺癌组和不典型增生组VIPR1 mRNA表达率依次为83.33%(20/24)、43.75%(14/32)和60.61%(20/33),3组间比较差异有统计学意义(P<0.05);VIPR2 mRNA表达率在正常对照组、胃腺癌组和不典型增生组依次为66.67%(16/24)、50.00%(16/32)和57.59%(19/33),3组间比较差异无统计学意义(P>0.05)。胃腺癌组的VIPR1mRNA的相对表达量均低于不典型增生组和正常对照组(P<0.01),而正常对照组和不典型增生组之间比较差异无统计学意义(P>0.05);VIPR2 mRNA的相对表达量在不同组间比较差异无统计学意义(P>0.05)。结论:胃腺癌组织中VIP mRNA的表达量高于正常胃黏膜和不典型增生胃黏膜组织,但VIPR1mRNA的表达量却低于正常胃黏膜和不典型增生胃黏膜组织,VIP可能与胃腺癌的发生发展有关。

Objective： To investigate mrna expression of vasoactive intestinal peptide and its receptor in gas- tric adenocarcinoma and understand the effect of VIP and VIPR on gastric adenocarcinoma and possible mechanism. Methods： Gastric tissues of 32 patients with gastric adenocarcinoma, 33 patients with dysplasia and 24 healthy controls were collected, and mrna expression of vasoactive intestinal peptide and its receptor in gastric adenocarcinoma were determined by real time PCR. Results： All of the gastric tissues among the three groups were positive in VIP mRNA expression. The levels of VIP mRNA in gastric adenocarcinoma was higher than that in dysplasia and controls （ P〈0.01）, while there was no difference between the dys-plasia and the rate of VIPR1 controls （ P〉0.05）. The mRNA was 83.33%（20/24 expression ） in gastric lower than 60.61% （20/ ：NA didn＇ t adenocarcinoma, which was significantly dysplasia 43.75% （14/32） and controls 60 33）（P 〈0.05）, but that of VIPR2 mRchanged significantly （66. 67% vs 50.00% and 57.59%） （ P 〉0.05）. The levels of VIPR1 mRNA in gas- tric adenocarcinoma was lower than that in dysplasia and controls （ P〈0.01）, while there was no differ- ence between the dysplasia and the controls （ P〉0.05）. There was no difference in the levels of VIPR2 mRNA among the three groups （ P〉0.05）. Conclusion： The expression of VIP mRNA in gastric adeno- carcinoma was higher than that in dysplasia and controls, while VIPR1 mRNA was lower than the two groups, so they might contribute to the diagnosis of gastric carcinoma. VIP might play an important role in the pathogenesis and progress of gastric adenocarcinoma.