黑龙江省马铃薯主栽品种遗传多样性的RAPD分析

RAPD Analysis of Genetic Diversity for Leading Potato Varieties in Heilongjiang Province

应用RAPD标记技术对黑龙江省34份马铃薯主栽品种的遗传多样性进行分析,明确这些主栽品种的亲缘关系,旨在为本区域马铃薯优良品种的选育提供理论依据。结果显示,34条随机引物共扩增出条带185条,其中多态性条带135条,占总条带数的73%;34份供试品种可聚为3大类7亚类,遗传距离在0.112~0.551之间,平均为0.364。结果表明,黑龙江省马铃薯主栽品种的遗传相似性较高,遗传基础比较狭窄。

The random amplified polymorphic DNA procedure （RAPD） was used to assess the genetic diversity for 34 leading potato varieties in Heilongjiang Province, which were to understand the genetic relationship of these leading potato varieties, and provide the theoretical basis for the breeding of potato varieties. The result showed that there were 135 polymorphic bands amplified with 34 primers, and the polymorphic rate was 73%;these 34 potato varieties were divided into three classes and seven subclasses, and the genetic distance among 34 potato varieties was ranged from 0.112 to 0.551, with an average of 0.364. These results suggest that the genetic similarity is high and the genetic base is narrow among leading potato varieties in Heilongjiang Province.