吡喹酮对斯氏肺吸虫损害的电镜和组织化学观察

HISTOLOGICAL AND HISTOCHEMICAL CHANGES OF PARAGONIMUS SKRJABINI INDUCED BY PRAZIQUANTEL

用吡喹酮60mg/kg 一次喂饲实验犬后72h 解剖观察。光镜可见虫体皮层有离散和脱落,白细胞浸入体表深处。睾丸、卵巢结构变疏松;透射电镜观察到虫体表皮坏死,皮下细胞膜不清,线粒体固缩。睾丸被膜内层结构不清,细胞核膜断续消失。卵巢细胞膜破损,核膜消失,核内有假包含体;组织化学观察到虫体糖原、核酸等均较服药前明显减少,唯碱性蛋白质溴酚蓝反应增强。

Praziquantel with a single dose of 60 mg/kg was given to dogs experimentally infected with Paragonimus skrjabini.72 hrs after treatment,the dogs were sacrificed and the worms collected and observed.The re- suits are as follows. Under optical microscopy,the integument of the worms showed degeneration and exfoliation with in- flammatory cells invading the parenchyma,testicle and ovary tissues revealed rarefaction in structure. Ultrastructural observation under transmission electron microscope showed that necrosis of integument, melting membranes of subintegument cell,discontinuity nucleus membranes of spermatogonia,as well as concentrated mitochondria and rupture oogonium membrane were presented.In addition,pseudoinclusion bodies could occasionally be found in the nucleus of oogonium. Histochemical changes showed glycogen,RNA and DNA were markedly decreased,but alkaline protein reaction of bromphenol blue was stronger than those in the control.