摘要
目的检测胃癌组织、相应癌旁组织及正常胃组织中ERCC1基因甲基化状态。方法采用甲基化特异性PCR(MSP)技术检测ERCC1基因甲基化。结果胃癌组织中ERCC1基因完全甲基化率为46.7%(14/30),部分甲基化率为30.0%(9/30),总甲基化率为76.7%(23/30),显著高于相应的癌旁组织中该基因的甲基化率13.3%(4/30)。10例正常胃组织中该基因未发生甲基化。结论胃癌组织中存在高比例的ERCC1基因甲基化。
Objective To detect the methylation status of ERCC 1 gene in gastric cancer tissues ,paired adjacent cancer-ous tissues and normal gastric tissues .Methods Methylation-specific PCR ( MSP) was used to detect the methylation of ERCC 1 gene.Results The complete methylation rate of ERCC1 gene in gastric cancer tissues was 46.7%(14/30),the partial methyla-tion rate was 30.0%(9/30),and the total methylation rate was 76.7%(23/30),which was significantly higher than the methy-lation rate in adjacent cancerous tissues (13.3%,4/30).There was no methylation in 10 cases of normal gastric tissues .Conclu-sion There is a high percentage of ERCC 1 gene methylation in gastric cancer tissues .
出处
《实用癌症杂志》
2014年第4期379-380,共2页
The Practical Journal of Cancer
关键词
胃癌
核苷酸切除修复交叉互补基因1
甲基化
甲基化特异性聚合酶链反应
Gastric cancer
Excision repair cross-complementing gene 1 ( ERCCI )
Methylation
Methylation specific polymerase chain reaction