胃癌ERCC1基因甲基化的研究

Study of Methylation of ERCC1 Gene in Gastric Cancer

目的检测胃癌组织、相应癌旁组织及正常胃组织中ERCC1基因甲基化状态。方法采用甲基化特异性PCR(MSP)技术检测ERCC1基因甲基化。结果胃癌组织中ERCC1基因完全甲基化率为46.7%(14/30),部分甲基化率为30.0%(9/30),总甲基化率为76.7%(23/30),显著高于相应的癌旁组织中该基因的甲基化率13.3%(4/30)。10例正常胃组织中该基因未发生甲基化。结论胃癌组织中存在高比例的ERCC1基因甲基化。

Objective To detect the methylation status of ERCC 1 gene in gastric cancer tissues ,paired adjacent cancer-ous tissues and normal gastric tissues .Methods Methylation-specific PCR （ MSP） was used to detect the methylation of ERCC 1 gene.Results The complete methylation rate of ERCC1 gene in gastric cancer tissues was 46.7%（14/30）,the partial methyla-tion rate was 30.0%（9/30）,and the total methylation rate was 76.7%（23/30）,which was significantly higher than the methy-lation rate in adjacent cancerous tissues （13.3%,4/30）.There was no methylation in 10 cases of normal gastric tissues .Conclu-sion There is a high percentage of ERCC 1 gene methylation in gastric cancer tissues .