3种提取全血基因组DNA的方法比较

Comparison of Three DNA Extraction Methods for Human Blood

通过比较提取全血基因组DNA的方法,从而获得一种易于在实验室推广的高效快速的DNA提取方法。本研究收集兰州大学第二医院神经内科90例住院患者抗凝血样,随机分为3组,分别使用硅胶膜吸附法、溶液盐析法及酚氯仿法提取全血基因组DNA。比较分析3种方法实验时间、提取DNA琼脂糖凝胶电泳结果和提取DNA的浓度及纯度,结果表明:3组实验时间比较结果显示,酚氯仿法耗时最长,溶液盐析法耗时最短;3组所提取DNA琼脂糖凝胶电泳结果显示,溶液盐析法DNA条带清晰、光密度高。3组提取DNA的浓度及吸光度比值比较显示,浓度由高到低依次为溶液盐析法、酚氯仿法、硅胶膜吸附法,差异具有统计学意义(浓度分别为43.99±18.89 ng/uL,30.29±16.31 ng/uL,22.62±3.65 ng/uL,p均<0.05),3组吸光度比值无统计学差异。因此,我们认为相比于酚氯仿法与硅胶膜吸附法,溶液盐析法是一种高效、便捷、快速的DNA提取方法。

We evaluated the performance of three DNA extraction methods in order to found an efficient and con- venient method to extract DNA from human blood in lab. In this study, we collected 90 blood samples of patients from neurology of the Second Hospital of Lanzhou University, which were divided into three groups, labeled as A, B and C group. Using three methods to extract DNA from blood samples respectively, silica membrane absorption for A group, salt fractionation for B group, and phenol chloroform for C group. We compared the time which taken on the methods, the results of agarose gel loading of DNA, the concentration and purity of DNA. The results showed that among the three methods, phenol chloroform method was the most time-consuming method. The re- sults of agarose gel loading of DNA showed that the lanes of DNA in B group were the most clear. The concentra- tion of DNA in B group is the highest among three groups. There was no significant difference on the purity of DNA among three groups. Compared with silica membrane absorption and phenol chloroform, we found that salt fractionation should be the most efficient and convenient method for DNA extraction from human blood.