不同工作环境PM2.5暴露对人群细胞因子的影响

Effects of different working environment PM2.5 exposure on cytokines in human being

目的探讨太原市外勤交警和疾控中心人员工作环境中PM2.5暴露对人群外周血细胞因子的影响,对深入阐明PM2.5的免疫毒性机制提供参考。方法选择太原市外勤交警为目标人群,以疾控中心(CDC)工作人员为对照,连续5个工作日监测各自工作环境中PM2.5,计算研究对象工作时间内PM2.5暴露剂量和潜在暴露剂量,以高效液相色谱法检测人群尿液1-羟基芘(1-OHPy)浓度作为内暴露标志物,用ELISA检测人群血清中细胞因子IL-1、IL-2、TNF-α水平。结果太原市外勤交警工作环境PM2.5浓度高于CDC工作环境PM2.5浓度(P<0.05);交警工作时间内的PM2.5暴露剂量、潜在暴露剂量和内暴露标志物均高于CDC人员(P<0.05)。交警血清中细胞因子IL-1和TNF-α表达水平高于CDC工作人员(P<0.05);而交警血清IL-2表达水平低于CDC工作人员(P<0.05)。且尿液中1-OHPy与血清中TNF-α呈正相关、与血清中IL-2呈负相关(P<0.05)。结论高暴露PM2.5可增加人群外周血IL-1、TNF-α表达,抑制IL-2分泌。

Objective To explore the effects of different working environment PM2.5 exposure on cytokines in human being.Methods Traffic polices were chosen as the target group, staffs of the CDC as the control group. The concentration of PM2.5 in their own working atmosphere was monitored, and working time PM2.5 exposure and potential exposure dose were calculated.Their peripheral blood was collected and lymphocyte and serum were separated. 1-OHPy in urine was measured by high performance liquid chromatography. Tumor necrosis factor（TNF-α）, interleukin-1（IL-1）, interleukin-2（IL-2） in serum were detected by ELISA. Results The concentration of PM2.5 in traffic polices＇ working atmosphere was higher than that in control group（0.132 mg/m3 versus 0.100 mg/m3）（P〈0.05）; The PM2.5 exposure dose, potential exposure dose and internal exposure dose of the traffic polices were higher than those of CDC staffs. The levels of IL-1 and TNF-α in serum of target group was significantly higher compared with control group（P〈0.05）, however, the level of IL-2 in serum of target group was lower than that in control group（P〈0.05）. 1-OHPy in urine was positively correlated with TNF-α and negatively correlated with IL-2 in serum. Conclusion High exposure to PM2.5 may increase the release of IL-1 and TNF-α in peripheral blood and suppress the secretion of IL-2.