贵州省间日疟原虫株的研究 Ⅲ.某些因素对红内期滋养体形态影响的观察

STUDIES ON PLASMODIUM VIVAX STRAIN IN GUIZHOU PROVINCE Ⅲ.OBSERVATION ON SOME FACTORS AFFECTING THE MORPHOLOGY OF ERYTHROCYTIC TROPHOZOITES

本文报告某些因素对温带株间日疟原虫红内期滋养体形态的影响。结果显示:(1)薛氏点的检出率与使用吉氏(Giemsa)染液的浓度密切相关,分别用2%、5%、7%、10%和15%染色,环状体期被寄生红细胞薛氏点的检出率分别为0%、0.3%、25.4%、18.0%和53.8%,阿米巴期薛氏点的检出率分别为0.7%、51.9%、69.8%、75.7%和88.1%;在同一例病人临床发作的发冷期、发热期和发作间歇期的薄血膜片,以同一浓度的吉氏液染色,薛氏点的检出率分别为35.4%、56.7%和88.3%。(2)来自疟区和无疟区的病人,无论长潜伏期或短潜伏期,均可查获多核疟原虫,来自疟区者检出率为1.68%,来自无疟区者为4.87%。(3)薄血膜片干燥快或慢与多核疟原虫和受染红细胞形态变化的检出率有显著性差异,用快速干燥法多核疟原虫与受染红细胞形态变化的检出率分别为1.47%和11.13%;用延迟干燥法则分别为3.3%和33.71%。

This paper reports that some factors affected the morphological change of erythrocyte trophozoites of Plasmodium vivax temperate strain in Guizhou Province.The results showed that:(1)The relationship be- tween detection rate of Schuffner's dots and concentration of Giemsa stain solution.The concentration of Giemsa stain solution used varied from 2%,5%,7%,10% to 15%,for the thin blood film,the detec- tion rates of Schuffner's dots in RBC infected with trophozoites were 0.7%,51.9%,69.8%,75.7% and 88.1% respectively,and that of RBC ring form were 0%,0.3%,25.4%,18% and 53.8% respective- ly;(2)In the same patient at the different stages of the disease-i.e.chills,fever and intermission stages, the detection rates of Schuffner's dots in RBC infected with trophozoites were 35.4%,56.7% and 88.3% respectively.Multinuclear trophozoites were discovered from the patients infected with plasmodium viuax tem- perate strain,no matter whether they came from malaria areas or malaria free areas.The detection rate of multinuclear trophozoites from the patients of malaria areas was 1.68% and 4.87% from the patients of malaria free areas;(3)The relationship between the method for drying the thin blood film and the detection rate of multinuclear trophozoites(RTM)and of infected erythrocyte morphological change(REMC).When the thin film was dried by the electric fan(fast drying method),the RTM and REMC were 1.47% and 11.13%,and the parasitized red cells were enlarged in 58.99%,only 29.88% the infected erythrocytes appeared normal.When the thin blood films were dried in the moist box(slow drying method),RTM and REMC were found to be 3.30% and 33.71% respectively,the infected erythrocytes enlarged accounted for 52.98%,and only 13.31% appeared normal.