转录组学及其在蔬菜学上应用研究进展

Advance in Transcriptomics and Its Application in Olericulture Research

介绍了转录组学的主要技术方法及其原理,并对转录组学在蔬菜及其他植物上的应用做了分析。认为研究转录组学可克服蔬菜基因组数据匮乏的障碍,弄清楚蔬菜特定基因的结构及其在抗逆、抗病等过程中的作用。研究表明,当前转录组学方法主要基于杂交技术或者测序技术,代表性方法有基因芯片、SAGE(serial analysis of gene expression)、MPSS(massively parallel signature sequencing)、RNA-Seq(RNA-sequencing)等;转录组学可用于了解蔬菜抗逆、抗病分子机制,证明特定基因表达情况,但是如何快速测序以及科学分析成为制约其发展的瓶颈因素。因此,研究得出转录组学在论证分子机制时需要谨慎,更需要借助蛋白组学、生物信息学等方法综合验证;但是,以转录组学为代表的前沿生物学理论应更多地应用于蔬菜学这门古老学科。

This article not only introduces the main method and principle of transcriptome but also analyzes itsapplication to vegetables and other plants. Genomic data scarcity existing in vegetables can be overcome by thestudy of transcriptome. What is more, it can clarify the genes structure and its effects in resistance. It wasshowed that the current method of transcriptome mainly based on hybridization or sequencing technologywhich consisted of gene chip, SAGE（serial analysis of gene expression）, MPSS（massively parallel signaturesequencing）, and RNA-Seq（RNA-sequencing）; research on transcriptome could be used to understand theresistance of vegetables, the molecular mechanism of disease resistance, and demonstrate specific geneexpression. But high-speed sequencing and scientific analysis were the bottlenecks restricting its development.Therefore, we believed that caution was needed in the molecular mechanism demonstration when proteomicsand bioinformatics were used to provide further evidence; while cutting- edge biological theories liketranscriptome should be applied more to the vegetables.