寒地粳稻低温发芽力和芽期耐冷性与SSR标记的关联分析

Association analysis of germinability under low temperature and cold tolerance at bud stage with SSR markers of japonica rice in cold region

以220份寒地粳稻品种资源进行低温发芽力和芽期耐冷性鉴定,利用118对SSR标记对低温发芽力和芽期耐冷性进行SSR标记关联分析。结果表明,品种间低温发芽力和芽期耐冷性存在明显差异。与相对发芽势、相对发芽率、相对发芽指数和成苗率相关联的SSR位点分别有4、2、3和4个。其中RM1220同时与低温发芽力的3个性状相关联,RM109和RM453同时与两个性状相关联,通过优异等位基因挖掘,筛选出一系列优异等位变异及相应载体材料,这些分子标记、优异等位变异和载体材料可为寒地粳稻耐冷育种提供有价值的信息和材料。

In order to investigate the germinability under low temperature and the cold tolerance at the bud stage of japonica rice, 220 varieties of japonica rice were used as materials and 118 pairs of SSR primes were selected. The results showed that there was a significant difference between varieties of the germinability under low temperature and the cold tolerance at the bud stage. The relative germination, relative germination rate, relative germination index and seedling rate associated SSR loci were 4, 2, 3 and 4, respectively. RM1220 was associated with three traits of the germinability under low temperature , RM109 and RM453 were associated with two traits, Through mining the superior al ele, we can screen a series of favorable al eles and the corresponding carrier material, which would provide valuable information and materials for breeding cold tolerance of japonica rice in cold region.