亚麻MAPK基因克隆及盐碱胁迫下的表达分析

Cloning and expression analysis of MAPK gene under saline-alkaline stress in flax

为研究MAPK基因与亚麻应答盐碱胁迫的关系,利用生物信息学方法从亚麻基因组中预测得到20个亚麻MAPK基因,命名为Lu MPK1~Lu MPK20,这20个MAPK基因都含有T[D/E]Y保守结构域,除Lu MPK5和Lu MPK20以外,其他基因都是两两一组。利用PCR技术克隆得到5个亚麻MAPK基因（Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14、Lu MPK16）,这5个基因序列与预测序列完全一致。利用数字基因表达谱数据,分析亚麻盐碱胁迫下这5个基因的表达情况。结果表明,这5个基因的表达均受到盐碱胁迫影响。Lu MPK3、Lu MPK11、Lu MPK14、Lu MPK16在中性盐胁迫下上调表达,Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14在碱性盐胁迫下上调表达,Lu MPK16在碱性盐胁迫下下调表达。研究为亚麻MAPK基因功能,尤其是耐盐碱功能研究奠定理论基础。

In order to investigate the relationship between flax MAPK genes and saline-alkaline stress, 20 flax MAPK genes, named LuMPK1-LuMPK20, repectively, were predicted from flax genome by bioinformatics methods. These 20 MAPK genes al contained T[D/E]Y conserved domains and existed in pairs except LuMPK5 and LuMPK20. Five MAPK genes （LuMPK3, LuMPK8, LuMPK11, LuMPK14, LuMPK16） were cloned by PCR and their sequences were consistent with the predicted sequences. The expressions of these genes were analyzed by digital gene expression under saline and alkaline stresses. The results showed that the expressions of these five genes were subject to saline and alkaline stresses. LuMPK3, LuMPK11, LuMPK14, LuMPK16 were up-regulated under neutral salt stress, and LuMPK3, LuMPK8, LuMPK11, LuMPK14 were up-regulated under alkaline salt stress. LuMPK16 was down-regulated under alkaline salt stress. This study laid the theoretical foundation for the function of MAPK genes in flax, especial y the saline-alkaline tolerance function.