钛基表面PDLLA/ZnO纳米阵列材料的制备及生物学性能

Preparation of PDLLA/ZnO Nanorod Arrays on Ti Substrate and the Biological Properties

采用水热生长法,在钛基表面制备高度有序、尺寸可控的氧化锌纳米棒阵列,再通过静电吸附的方法在其表面自组装一层具有良好生物相容性的聚乳酸生物涂层。利用SEM,MTT,LDH方法对细胞形态和生物活性进行表征。结果表明,氧化锌阵列上细胞形态呈圆形,无铺展现象。而经聚乳酸修饰过的材料表面MC3T3细胞外基质铺展较好,细胞分泌的丝状伪足嵌入了阵列结构的空隙中,并且表面细胞数量最多,细胞活性明显高于氧化锌纳米棒及纯钛片。结果表明,聚乳酸涂层提高了氧化锌阵列的生物相容性,为细胞生长提供适宜的微环境,有效促进了细胞的黏附与增殖。

Highly ordered and size controllable Zn O nanorod arrays were prepared on Ti substrate through a hydrothermal method, and then the biocompatible PDLLA coating was self-assembled onto the surface of the prepared nanorod arrays via electrostatic adsorption process. SEM, MTT and LDH methods were used to characterize the morphology and biological activity of cells. The results show that cells on the Zn O nanorod arrays are round-like without spreading; while after the modification of PDLLA, there are the largest amount of cells on the modified nanorod arrays, the extracellular matrix become well-spread with many filopodia embedded into the gaps of the array structure, and the cell activity is significantly higher than that of Zn O nanorod arrays and titanium film. It is concluded that the PDLLA coating can improve the biocompatibility of the material to provide a suitable microenvironment for cell growth, and effectively promote the adhesion and proliferation of cells.