过量氟对小鼠糖代谢的影响

The effects of excessive fluoride on glucose metabolism in mice

目的 观察不同剂量染氟对C57小鼠糖代谢的影响.方法 选取40只雄性C57小鼠,按体质量（20～24 g）采用随机数字表法分为对照组,50、100、150 mg/L染氟组,每组10只小鼠.染氟组大鼠分别给予含50、100、150 mg/L氟化钠的蒸馏水,对照组饮用超纯水.在染氟后第2、4、6、8、10、12周,对各组大鼠进行体质量检测,血糖仪和糖化血红蛋白仪检测血糖和血清糖化血红蛋白含量,酶联免疫吸附试验（ELISA）检测血清胰岛素、胰高血糖素含量.结果 染氟第10、12周,各组间C57小鼠空腹血糖比较差异均有统计学意义（F=35.12、21.92,P均＜0.05）,其中100、150 mg/L染氟组小鼠空腹血糖[（7.7±0.2）、（7.3±0.3）、（8.6±0.5）、（9.1±0.7）mmol/L]均高于对照组[（5.4±0.3）、（5.0±0.3） mmol/L,P均＜0.01].各组间C57小鼠血清糖化血红蛋白、胰高血糖素含量比较差异均有统计学意义（F=3.85、8.74,P均＜0.05）.其中100、150 mg/L染氟组小鼠血清糖化血红蛋白[（7.73±0.76）、（7.80±1.15） mmol/L]均高于对照组[（5.43±1.27） mmol/L,P均＜0.05];50、100、150mg/L染氟组小鼠血清胰高血糖素含量[（19.15±11.84）、（26.55±15.97）、（20.05±7.29）ng/L]均低于对照组[（48.35±2.79）ng/L,P均＜0.01].结论 长期摄入过量的氟,能降低C57小鼠的糖代谢.

Objective To observe glucose metabolism in C57 mice treated with different doses of fluoride.Methods Forty male C57 mice （body weight 20-24 g） were divided into four groups which were exposed to 0,50,100 and 150 mg/L sodium fluoride （NaF） by random number table according to body weight,each group had 10 mice.At 2,4,6,8,10 and 12 weeks after fluoride exposure,body weight was measured,blood glucose and glycosylated hemoglobin were detected by blood glucose meter and glycosylated hemoglobin meter,serum insulin and glucagon were detected by enzyme-linked immunosorbent assay （ELISA）.Results At 10 and 12 weeks after fluoride exposure,the differences of fasting glucose between groups of C57 mice were statistically significant （F =35.12,21.92,all P ＜ 0.05）,the fasting glucose of 100,150 mg/L fluoride groups [（7.7 ± 0.2）,（7.3 ± 0.3）,（8.6 ± 0.5）,（9.1 ± 0.7）mmol/L] were higher than those of the control group [（5.4 ± 0.3）,（5.0 ± 0.3）mmol/L,all P ＜ 0.01].The differences of glycosylated hemoglobin,glucagons between groups were statistically significant （F =3.85,8.74,all P ＜ 0.05）.The glycosylated hemoglobin of 100,150 mg/L fluoride groups [（7.73 ± 0.76）,（7.80 ± 1.15） mmo]/L] were higher than those of the control group [（5.43 ± 1.27） mmol/L,all P ＜ 0.05]; serum glucagon levels of 50,100,150 mg/L fluoride groups [（19.15 ± 11.84）,（26.55 ± 15.97）,（20.05 ± 7.29）ng/L] were lower than that of the control group [（48.35 ± 2.79）ng/L,all P ＜ 0.01].Conclusion Long term excess fluoride intake can reduce the function of sugar metabolism in C57 mice.