清热化湿舒胃方对幽门螺旋杆菌感染小鼠体内NO和胃组织iNOS表达的影响

Effects of Qingre Huashi Shuwei Fang on serum nitric oxide and expression of inducible nitric oxide synthase in gastric tissue in mice with Helicobacter pylori infection

目的:观察清热化湿舒胃方对KM小鼠感染幽门螺旋杆菌(Helicobacter pylori,H.pylori)动物模型血清中一氧化氮(nitric oxide,NO)含量和胃组织中诱导性一氧化氮合酶(inducible nitric oxide synthase,iNOS)表达的影响.方法:在复制KM小鼠H.pylori感染模型的基础上,分为清热化湿舒胃方组、三联抗生素治疗组、清热化湿舒胃方结合三联抗生素组和模型对照组,并分别进行干预和治疗.治疗结束后采用硝酸还原酶法测定各组小鼠血清中的NO含量,采用免疫组织化学法测定各组小鼠胃组织中iNOS的表达量.结果:所建立的H.pylori感染小鼠模型出现H.pylori在胃组织大量定植并造成胃黏膜慢性炎症损伤,体内NO含量和胃组织iNOS表达显著升高;清热化湿舒胃方干预后胃黏膜慢性炎症评分、体内NO含量和胃组织iNOS表达显著降低,其与三联抗生素联用对H.pylori的根除效果优于单用三联抗生素.结论:清热化湿舒胃方具有修复H.pylori感染所致胃黏膜炎症损伤的作用,其机制可能与降低胃组织iNOS活性表达和体内NO释放有关.

AIM： To observe the effects of Qingre Huashi Shuwei Fang on the content of nitric oxide（NO） in blood and the expression of inducible nitric oxide synthase（i NOS） in gastric tissue in mice with Helicobacter pylori（H. pylori） infection. METHODS： After the successful induction of H. pylori infection in mice, the model mice were treated with Qingre Huashi Shuwei Fang, triple therapy（amoxicillin, esomeprazole sodium and clarithromycin）, or Qingre Huashi Shuwei Fang combined with triple therapy. Non-treated model rats or normal rats were used as controls. After treatment, the content of NO in blood was determined by the Griess method, and the expression of i NOS in gastric tissue was detected by immunohistochemistry. RESULTS： In the model group, chronic inflammation in gastric tissue was observed, and the content of NO in blood and the expression of i NOS in gastric tissue increased significantly. The content of NO in blood and the expression of i NOS were significantly decreased in the Qingre Huashi Shuwei Fang group. The H. pylori eradication in the Qingre Huashi Shuwei Fang combined with triple therapy group was better than that in the triple therapy group. CONCLUSION： Qingre Huashi Shuwei Fang can reduce gastric mucosal injury caused by H. pylori infection, and this may have a relationship with its negative effect on the expression and release of i NOS in gastric tissue.