葛根素对创伤性脑损伤的保护作用

Protective effects of puerarin on traumatic brain injury

目的探讨葛根素对创伤性脑损伤的保护作用。方法选择健康成年雄性SD大鼠,构建创伤性脑损伤动物模型,40只大鼠随机分为四组,损伤组(A组)和假手术组(B组),葛根素治疗的损伤组(C组)和葛根素治疗的假手术组(D组)。实验以神经功能缺损评分(m NSS)和脑组织干湿重比来评估创伤性脑损伤的损伤情况,并检测氧化应激产物MDA的水平。结果与A组相比,B组和C组均出现了神经功能缺损评分增高、脑干湿重比增加,脑组织中氧化应激产物MDA含量增加。而与B组相比,C组葛根素能显著降低神经功能缺损评分和脑干湿重比,也能减少氧化应激产物MDA的水平。结论葛根素对创伤性脑损伤具有保护作用。

Objective To explore the neuroprotection of puerarin on traumatic brain injury. Methods Establish experimental animal model of traumatic brain injury by using SD rats, 40 rats were randomized into four groups, traumatic brain injury group （group A）, sham group （group B）, Puerarin treatment group （group C） and sham group with Puerarin treatment（group D）. Modified Neurological Severity Score （mNSS） and Brain edema were measured to evaluate the neuroprotection of Puerarin on traumatic brain injury. The level of MDA in brain were also tested by ELISA. Results Comparing with group A, mNSS and Brain edema of rats in group B increased after traumatic brain injury. Meanwhile, mNSS and brain edema of rats in gourp C were attenuated by puerarin compared with group B, and the level of MDA declined after the treatment of puerarin. Conclusion Puerarin could improve the traumatic brain injury.