气相色谱法测定止汗搽剂中苯酚和乙醇的含量

Determination of phenol and ethanol in antihidrotic liniment by gas chromatography

目的：建立测定止汗搽剂中苯酚和乙醇含量的气相色谱法。方法采用HP-5毛细管柱（30m×0.53mm，5.0μm），载气为氮气，进样口温度220℃，FID检测器温度250℃；初始柱温90℃，保持6min，以20℃/min的速率升至170℃，保持7min；分流比10∶1；溶剂为N，N-二甲基甲酰胺。结果苯酚和乙醇的线性范围分别为0.0497～1.59mg/mL和0.0753～2.41mg/mL，回归方程分别为A=5869C-73.487（r=0.9998）和A=2561.6C-9.5617（r=0.9999）。苯酚和乙醇的平均回收率分别为102.8%（RSD=1.44%）和101.4%（RSD=1.45%）。结论该方法简便、快捷，灵敏度高，准确度好，可用于止汗擦剂中苯酚和乙醇的含量测定。

[Abartact] Objective To establish a GC method for the content determination of phenol and ethanol in antihidrotic liniment. Methods Phenol and ethanol in antihidrotic liniment were separated by HP-5 capillary column（30.0m×0.32mm,5.0μm）with the FID detector.The carried gas was nitrogen.The injector temperature and detector temperature was controlled at 220℃and 250℃,respectively.The column temperature was maintained at 90℃for 6 min,then raised at a rate of 20℃per min to 170℃and maintained 7 min.The splitting-ratio was 10：1.The sample was dissolved in N,N-dimethyl formamide. Results The linear range of phenol and ethanol were 0.0497-1.59mg/mL and 0.0753-2.41mg/mL.Regression equations were A=5869C-73.487（r=0.9998）and A=2561.6C-9.5617（r=0.9999）. The average recovery of phenol and ethanol were 102.8%（RSD=1.44%）and 101.4%（RSD=1.45%）. Conclusion The eatablished method is simple,rapid,sensitive,accurate and reliable which can be used for determination of phenol and ethanol in antihidrotic liniment.