高效液相色谱法测定人血浆中依托咪酯的浓度

Determination of etomidate concentration in plasma by HPLC

目的：建立测定人血浆依托咪酯浓度的HPLC方法。方法色谱柱为AgilentTC-C18柱（250mm×4.6mm，5μm），流动相为甲醇-水（75：25），流速为1.0mL/min，检测波长为241nm。结果依托咪酯浓度在0.25～5.00μg/mL范围内与峰面积呈良好线性关系（r=0.9987），方法回收率在98.6%～101.3%，提取回收率在72.6%～74.6%。结论本方法操作简便、准确、可靠，适用于测定人血浆中依托咪酯的浓度。

Objective To develop a method for the determination of etomidate concentration in plasma by using HPLC and to provide scientific analysis for the studies of etomidate clinical pharmacology. Methods The Agilent TC-C18 （250mm×4.6mm,5μm） was used as analytical columu with mobile phase consisted of methanol and water （75：25） .The flow rate was 1.0 mL/min and detection wavelength was 241nm. Results The linear ranges were 0.25-5.00μg/mL for etomidate in human plasma.The method recover were 98.6%-101.3%and the extraction recovery were 72.6%-74.6%. Conclusion The method is simple, accurate and reliable which is suitable for the determination of etomidate in human plasma.