根癌农杆菌介导的地衣型真菌Cladonia metacorallifera的转化

Agrobacterium tumefaciens-mediated transformation of the lichen forming fungus Cladonia metacorallifera

以潮霉素抗性和增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)作为筛选标记,利用地衣型真菌Cladonia metacorallifera的菌丝,成功实现了根癌农杆菌介导的遗传转化,PCR检测证明转化子中存在潮霉素抗性基因,共聚焦显微镜检测到转化子菌丝能够产生绿色荧光,证明EGFP能够在trp C启动子控制下在地衣型真菌中表达。

Agrobacterium tumefaciens-mediated transformation （ATMT） was successfully applied to Cladonia metacorallifera. Vegetative mycelium of this fungus was used for transformation by ATMT. resistance （hph） and the enhanced green fluorescent protein （EGFP as selection markers. PCR amplification of hph and the observation metacorallifera was successfully transformed by ATMT. the lichen forming fungus The genes for hygromycin ）, both under the control of a trpC promoter, were employed of fluorescence signal by confocal microscopy showed that C.