microRNA－10a－5p在喉癌前病变组织中的表达

Expression of microRNA-10a-5p in laryngeal epithelial premalignant lesions

目的：探讨microRNA－10a－5p（miR－10a）在喉癌前病变（LEPL）组织中表达的差异，并结合临床因素进行病理分析。方法参考WHO头颈部肿瘤病理学和遗传学（2005）分类系统，运用实时定量聚合酶链式反应方法检测miR－10a在94例LEPL组织中[轻度异型增生（ MID）、中度异型增生（ MOD）、重度异型增生（ SD）及原位癌（ CIS）]的表达，并比较各组间的表达差异，结合临床资料及随访结果分析miR－10a与各组别LEPL患者临床因素的相关性。结果随着LEPL异型程度的增加， miR－10a在LEPL组织中呈下调表达趋势，且在低风险病变组（ MID＋MOD）与高风险病变组（ SD＋CIS）之间的表达差异具有统计学意义（P＝0．03）；线性回归分析显示miR－10a与LEPL患者的性别、组织异型增生的程度存在线性相关（ P＜0．05）。结论 miR－10a在不同级别LEPL组织中的表达呈下调趋势，可为LEPL病理分级和早期喉癌诊断提供辅助参考。

Objective To investigate the expression of microRNA-10a-5p （ miR-10a） in laryngeal epithelial premalignant lesions （ LEPL ） and to analyze the correlations of its dys-regulation with clinicopathologic parameters of LEPL.Methods According to the WHO classification （2005）, 94 cases of LEPL were grouped into mild dysplasia （MID）, moderate dysplasia （MOD）, severe dysplasia （SD） and carcinoma in situ （CIS）.The expression of miR-10a in 94 cases of LEPL was detected by quantitative real-time polymerase chain reaction （ qRT-PCR） , and correlated with the clinical and follow-up data of all LEPL patients.Results miR-10a was down-regulated in LEPL with increasing grade of dysplasia.There was significantly statistical difference between low-risk （ MID＋MOD） and high-risk （ SD＋CIS） lesion groups （ P=0.03）.The linear regression analysis showed that miR-10a was correlated with grade and gender of LEPL （P〈0.05）.Conclusions Down regulation of miR-10a may be a useful molecular marker for grading of LEPL and diagnosis of early laryngeal cancer.