贵州省97家临检室阴道分泌物常规检验方法及质控情况调研

Investigation of leucorrhea routine examination methods and quality control of 97 clinical laboratories in Guizhou Province

目的调查贵州省医疗机构临床检验实验室阴道分泌物常规检验方法及质控（QC）情况。方法现况调查。于2009年随机抽取贵州省97家实验室，调查阴道分泌物常规检验方法和室内质控（IQC）开展情况，同时，向参与调研的实验室进行IQC措施重要性的宣教。于2011年再次对相同实验室的相同内容调研，并对两次调查检验方法和IQC变化情况进行x^2检验。结果2009年与2011年相比，仅采用生理盐水悬浮法进行阴道分泌物检验的实验室分别有16家与17家（）（2=0．037，P〉0．05）；同时用生理盐水和10％KOH悬浮法的实验室分别有16家与2家（X^2=12．003，P〈0．01）；采用染色法的实验室分别有64家与79家（X^2=5．488，P〈0．05）；采用悬浮法结合染色法检测的实验室分别有60家与73家（x^2=4．041，P〈0．05），其中以生理盐水悬浮法结合瑞氏和革兰染色检测的实验室分别有3家与28家（X^2=23．996，P〈0．01）；采取IQC措施的实验室分别有2家与88家（X^2=153．293，P〈0．01）。结论本次调研实验室中检测阴道分泌物以悬浮法为主。通过宣教可提高检验质量。

Objective To investigate the methods and internal quality control （IQC）leucorrhea routine examinationin clinical laboratories of medical institutions in Guizhou Province. Methods In 2009, 97 clinical laboratories were randomly selected for the first investigation. At the same time, staffs in theinvestigated laboratories were educated on the importance of IQC. The second investigation of the same items was carried out in 2011 inthe same laboratories. The results of the two investigations were analyzed byChi-square test. Results 2009 and 2011 numbers of laboratories thoseonly used normal saline suspension method for leucorrhea examination were 17and 16 （ X^2 = 0. 037, P 〉 0.05 ） respectively, used bothnormal saline and 10% KOH suspension methodswere 16and 2 （ X^2 = 12. 003, P 〈 0.01 ）, used staining method were 64and 79 （ X^2 = 5. 488,P 〈 0.05 ）, both used suspension and staining methods were 60and 73 （ X^2 = 4. 041, P 〈 0.05）, used normal salinesuspension method combined with Wright stain and Gram staining methods were3and 28 （ X^2 = 23. 996, P 〈 0.01 ） respectively. Numbers of Laboratoriespracticing IQC were 2and 88in 2009 and 2011 respectivly （ X^2 = 153. 293, P 〈 0.01 ）. Conclusions Currently, the most common used method for leucorrhea routine examination is suspension. Through the investigations and education, the quality ofleueorrhea routine examination was improved in Guizhou Province. （ Chin J Lab Med,2015 ,38 ：199- 202）