2008-2013年宁夏小肠结肠炎耶尔森菌分布特征分析

Characteristics of Yersinia enterocoliticain Ningxia,China,2008-2013

目的了解宁夏小肠结肠炎耶尔森菌的分布特征。方法按照《全国小肠结肠炎耶尔森菌病监测方案》对宁夏2008-2013年采集的各类样本进行分离培养,将分离株送中国疾病预防控制中心传染病所进行血清分型、毒力基因检测和脉冲场凝胶电泳(PFGE)分型。结果采集各类样本9 643份,检出173株小肠结肠炎耶尔森氏菌,总检出率1.79%;各年度和各样本检出率差异均有统计学意义(P<0.01)。血清型O∶3和O∶9均携带ail和ystA基因,全部从猪和腹泻病人样本中检出;血清型O∶5、O∶8和未分型菌株均没有携带ail和ystA基因,在猪、牛、羊、鸡和狗样本中都有检出。结论小肠结肠炎耶尔森菌在宁夏分布较为广泛,猪是主要宿主,流行的致病性血清型以O∶3为主,O∶9为辅,且在我区的分布没有时间、地域上差异。

We investigated the distribution characteristics of Yersinia enterocolitica in Ningxia ,China .In accordance with the requirements of the National Yersinia enterocolitica Disease Monitoring Scheme ,Y .enterocolitica were isolated from differ‐ent kinds of specimens collected in Ningxia in 2008 to 2013 .Then they were serotyped and detected for virulence gene and ana‐lyzed the pulsed‐field gel electrophoresis （PFGE） in Chinese CDC .It was found that 173 strains were isolated from various types of 9 643 specimens ,and the detection rate was 1 .79% .There were statistical differences among detection rates in differ‐ent years and in different specimens （P〈0 .01） .Pathogenic serotypes O∶3 and O∶9 carried ail gene and ystA gene were de‐tected from specimens of pigs and diarrhea patient .Non‐pathogenic serotypes O∶5 and O∶8 and non‐typeable strains didn＇t carry ail gene and ystA gene ,and also can＇t be detected from swine ,cattle ,sheep ,chickens and dogs .In conclusion ,Y .en‐terocolitica was widely distributed in Ningxia and pigs were the dominant animal host .In all pathogenic serotypes ,the highest proportion was O∶3 following by O∶9 .It was no time and regional difference in the distribution of that in Ningxia ,China .