2009-2012年登封市小肠结肠炎耶尔森菌分布调查

Investigation on distribution of Yersinia enterocolitica in Dengfeng from 2009 to 2012

目的调查登封地区家畜家禽小肠结肠炎耶尔森菌感染状况。方法采集家畜家禽新鲜粪便分离小肠结肠炎耶尔森菌,对分离菌株进行生化鉴定、血清分型、生物分型及毒力基因检测。结果从1 285份粪便标本中共检出105株,检出率8.17%。其中狗17株(17.35%);猪35株(13.62%);检出O∶3血清型12株(13.48%),O∶5血清型12株(13.48%),O∶8血清型14株(15.73%);Ail+、ystA+、yadA+、virF+的菌株占12.36%,ystB+的菌株占42.70%。结论登封市家禽家畜普遍携带小肠结肠炎耶尔森菌,主要动物宿主为猪和狗,是小肠结肠炎耶尔森菌人兽共患感染性腹泻病的重要传染源。

To investigate the infection of Yersinia enterocolitica in Dengfeng City ,the strains were isolated from livestock and poultry .The strains were detected with biochemiological methods ,serological methods ,and virulence genes were detected with PCR .A total of 105 strains of Yersinia enterocolitica were classified from 1 285 stool samples ,the total isolation rate was 8 .17% .Among the total isolated strains ,17 strains were classified from dogs with a rate of 17 .35% and 35 strains from pigs with 13 .62% .Twelve strains were O ∶3 serotype （13 .48% ） ,12 strains were O ∶5（13 .48% ） ,and 14 strains were O ∶8 （15 .73% ） .Ail＋ ,ystA＋ ,yadA＋ and virF＋ accounted for 12 .36% ,and ystB＋ accounted for 42 .70% .In conclusion ,the pigs and dogs were important animal hosts ,which may play the major role in humans＆#39;infection .