微粒体环氧化物水解酶基因多态性与膀胱移行细胞癌发生的关系

Association of gene polymorphism in microsomal epoxide hydrolase and susceptibility to bladder transitional cell carcinoma

目的探讨微粒体环氧化物水解酶(mEH)基因型与膀胱移行细胞癌(BTCC)的相关性。方法应用聚合酶链反应-限制性片段长度多态性分析方法检测BTCC患者(BTCC组,216例)和健康人群(对照组,300例)mEH基因型频率的分布。设计流行病学调查问卷进行面访调查。结果 BTCC组野生型、杂合型和变异型mEH第3外显子(mEH-exon3)分别占60.6%、27.3%和12.0%,对照组分别占46.3%、39.0%和14.7%(P<0.05);杂合型mEH-exon3为BTCC发生的保护性因素(OR=0.56,95%CI=0.41-0.82)。BTCC组野生型、杂合型和变异型mEH第4外显子(mEH-exon4)分别占85.6%、13.4%和0.9%,对照组分别占82.3%、15.0%和2.7%(P>0.05)。吸烟的mEH-exon3野生型人群的BTCC风险高于不吸烟者(P<0.05)。结论 mEH-exon3非野生型可能是BTCC发生、发展中的保护性因素之一;吸烟为BTCC发生的重要危险因素。而mEH-exon4基因多态性和BTCC的发生无明显相关性。

Objective To investigate the correlation between microsomal epoxide hydrolase（mEH）gene and the susceptibility to bladder transitional cell carcinoma（BTCC）.Methods With PCR-RFLP technique,the genotypes of mEH polymorphisms were analyzed in 216 BTCC patients（group A）and 300 matched healthy people（group B）.The epidemiological investigations were performed by questionnaire.Results The percentages of the 3rd mEH exon（mEH-exon3）in group A were 60.6%,27.3% and 12.0%for wild type,hybrid type and variant,respectively,which in group B were 82.3%,15.0% and 2.7%（P〈0.05）.The hybrid type mEH-exon3 was a protective factor for the occurrence of BTCC（OR=0.56,95% CI=0.41-0.82）.The percentages of the 4th mEH exon（mEH-exon4）in group A were 85.6%,13.4% and 0.9%,respectively,which in group B were82.3%,15.0% and 2.7%（P〉0.05）.In people with mEH-exon3 wild type,the risk for BTCC was higher in smokers than that in nonsmokers（P〈0.05）.Conclusion The non-wild type mEH-exon3 might be one of the protective factors for the occurrence and development of BTCC.Smoking is an important risk factor for the occurrence of BTCC.There is no significant association between gene polymorphisms and the occurrence of BTCC.