人类乳头状瘤病毒DNA及其亚型在喉咽癌中的检测

Expression of HPV DNA and its different subtypes in laryngopharyngeal carcinoma

目的通过检测喉咽鳞状细胞癌(喉咽癌)中人乳头状瘤病毒(HPV)的DNA及其亚型的表达,探讨HPV感染与喉咽癌的关系及在喉咽癌发病中的意义。方法采用聚合酶链式反应(PCR)扩增和DNA反向点杂交相结合的DNA芯片技术,检测36例喉咽鳞状细胞各亚型HPV DNA的表达。对照组为35例喉咽黏膜细胞,其中取自声带息肉患者26例,会厌囊肿患者9例。结果喉咽癌组36例中HPV DNA检出者6例,HPV DNA检出率16.7%(6/36)与对照组(0%)比较,差异具有统计学意义(χ2=4.40,P=0.036,P<0.05)。喉咽癌组高危型HPV DNA检出者与对照组比较,差异无统计学意义(χ2=3.323,P=0.068,P>0.05)。喉咽癌组低危型HPV DNA检出者与对照组比较,差异无统计学意义(P=1.00,P>0.05)。结论本研究观察到HPV感染与喉咽鳞癌有关,HPV DNA检出率为16.7%;HPV DNA阳性的喉咽鳞癌患者中以高危型HPV为主,占83.3%。

Objective To investigate the role of human papillomavirus（ HPV） in the development of laryngopharyngeal squamous cell carcinoma by detecting the expression of HPV DNA and its different subtypes.Methods36 patients with laryngopharyngeal carcinoma were enrolled.And 35 patients including 26 of vocal cord polyp and 9 of epiglottic cyst were chosen as control group.During operation,laryngopharyngeal mucosal cells were collected in all cases with cotton swabs.Expressions of HPV subtypes were detected in both groups with DNA chip technology combining polymerase chain reaction（ PCR） with DNA reverse point hybrid.Results Expression of HPV DNA was detected in 6 of the 36 laryngopharyngeal carcinoma specimens（ with an expression rate of 16.7%） and none of the control specimens（ 0%）.The difference was significant（ P〈0.05）.High-risk HPV DNA was detected in 5 specimens of laryngopharyngeal group,and the difference of detective rate between the laryngopharyngeal group and the control group was statistically insignificant（ P〉0.05）.Low-risk HPV DNA was detected in one specimen of laryngopharyngeal group,and the difference was also insignificant（ P〉0.05）.Conclusions HPV infection may be closely related with laryngopharyngeal squamous cell carcinoma.High-risk HPV makes up 83.3%,the majority,of the HPV-positive laryngopharyngeal carcinoma patients.