类1型-趋化因子配体3和趋化因子配体4基因与艾滋病患者抗病毒治疗后免疫重建的相关性

The correlation between human chemokine type 1 chemokine ligand 3 and chemokine ligand 4 gene and the immune reconstitution of acquired immunodeficiency syndrome patients after antiretroviral therapy

目的 探讨艾滋病患者血清类1型-趋化因子配体3 （CCL3L1）和趋化因子配体4（CCL4L）基因与抗病毒治疗后免疫重建的相关性.方法 实时定量PCR检测217例艾滋病患者接受抗病毒治疗前CCL3L1和CCL4L基因的表达水平,并与CD4＋和CD8＋T淋巴细胞计数作相关性分析.CD4＋和CD8＋T淋巴细胞变化值定义为开始抗病毒治疗后48个月内的月均变化值,变化率定义为抗病毒治疗后48个月时的数值与开始抗病毒治疗前数值比的对数值.组间比较采用方差分析.结果 CCL3L1基因中位数为2（0～8）拷贝,CCL4L基因中位数为3（0～7）拷贝.抗病毒治疗后,在CCL3L1基因为4～8拷贝、2～3拷贝和0～1拷贝组CD8＋T淋巴细胞变化值（F=3.054,P＜0.05）、CD4＋/CD8＋T淋巴细胞变化率（F=3.520,P＜0.05）差异均有统计学意义.CCL3L1基因0～1拷贝组与4～8拷贝组间CD8＋T淋巴细胞计数变化值（P=0.023）和变化率（P=0.038）差异有统计学意义.CD4＋/CD8＋T淋巴细胞变化率在CCL3L1基因为4～8拷贝与2～3拷贝组（P=0.010）、0～1拷贝组（F=4.397,P＜0.05）差异有统计学意义.CD4＋/CD8＋T淋巴细胞变化率在CCL4L为4～7拷贝组与0～2拷贝组间（P=0.030）、3拷贝组间（P=0.005）差异有统计学意义.随着CCL4L表达由低到高,CD4＋/CD8＋T淋巴细胞比值变化值随之递增.结论 CCL3L1和CCL4L基因表达可在一定程度上预测艾滋病患者接受抗病毒治疗后免疫重建的能力.

Objective To investigate the correlation between the human chemokine type 1 chemokine ligand 3 （CCL3L1） and chemokine ligand 4 （CCL4L） gene expression and the immune reconstitution of acquired immunodeficiency syndrome （AIDS） patients after antiretroviral therapy.Methods The gene copy numbers of CCL3L1 and CCL4L were detected by real time polymerase chain reaction in 217 AIDS patients before antiretroviral therapy.And the correlation between CCL3L1 and CCL4L gene copy numbers and the level of CD4＋ and CD8＋ T lymphocytes were analyzed.The changes of CD4＋ and CD8＋ T lymphocytes were defined as mean change value per month after 48 months treatment.The change rates of CD4＋ and CD8＋ T lymphocytes were defined as the logarithm of the ratio of the value after 48 month to that at baseline.Comparison between groups was conducted using analysis of variance.Results The median of gene copy numbers of CCL3L1 and CCL4L were 2 （range：0-8） and 3 （range：0-7）,respectively.After antiviral treatment,there were significantly different changes of CD8＋ T lymphocyte level （F=3.054,P〈0.05） and change rate of CD4＋/CD8＋ （F=3.520,P〈0.05） among groups of high （gene copy 4-8）,median （gene copy 2-3） and low （gene copy 0-1） CCL3L1 gene copy numbers.The changes of CD8＋ T lymphocyte levels （P=0.023） and change rates （P=0.038） in high and low CCL3L1 gene copy groups were both significantly different.There were significant differents changes rate of ratio of CD4＋/CD8＋ T lymphocyte among high and median （P=0.010）,high and low CCL3L1 gene copy numbers （F=4.397,P〈0.05）.The significant difference of the change rates of CD4＋/CD8＋ were found between the gene copy 3 group and gene copy 4-7 group CCL4L （P=0.005） and between the gene copy 4-7 group and gene copy 0-2 group of CCL4L （P=0.030）.The change ratio of CD4＋/CD8＋ T lymphocytes increased with the increase of copy numbers of CCL4L gene.Conclusions The gene expressions of CCL3L1 and CCL4L might be associated with the ability of immune reconstitution of AIDS patients after antiretroviral therapy.