微生物卤代烷烃脱卤酶研究进展

Research progress of microbial haloalkane dehalogenase-A review

卤代烷烃脱卤酶是降解卤代脂肪族化合物的关键酶类,在各种地理环境中的不同微生物中广泛存在,在生物降解和工业生产等方面具有重要的应用价值。目前已经生化鉴定了20个卤代烷烃脱卤酶。近些年来对这些酶的酶学特征、蛋白质结构和系统进化进行了详细的研究。同时,为满足应用实践的需求还对卤代烷烃脱卤酶进行了蛋白质工程改造研究。本文将对卤代烷烃脱卤酶研究的一些新的进展进行综述。

Haloalkane dehalogenases are key enzymes for biodegradation of halogenated aliphatic compounds,widely distributed in various microbial species of wide geographical distributions,and of significance in practical application such as bioremediation and industrial biocatalysis. Twenty haloalkane dehalogenases have been biochemically characterized so far. In recent years,progresses have been made in the enzymatic characteristics,protein structures,and phylogenetic diversity. We reviewed the progresses of haloalkane dehalogenase in structure and function,diversity and application potential.