摘要
目的:探讨心力衰竭时心肌细胞肌浆网钙回摄异常的机制。方法:结扎大鼠冠状动脉左前降支制备慢性心衰模型。4周后采用酶解法分离假手术组和心衰组大鼠心室肌细胞,利用激光扫描共聚焦显微镜检测心肌细胞肌浆网钙含量。采用western-blot技术检测心肌细胞肌浆网钙调蛋白SERCA2a、PLN、PP1的表达水平。结果:心衰组的大鼠心肌细胞荧光强度小于假手术组,并且心衰组大鼠心肌细胞咖啡因诱导的钙瞬变也低于假手术组;心衰组大鼠心SERCA2a表达水平降低,PP1的水平升高,PLN总体水平不变,但磷酸化的PLN水平降低。结论:钙泵表达量下降和钙泵活性降低是心力衰竭时心肌细胞肌浆网钙回摄减少的主要原因。
To explore the mechanism of sarcoplasmic reticulum calcium uptake in heart failure myocytes. Methods： Adult rat ventricular myocytes were isolated by enzymatic digestion from the rat model of chronic heart failure, which was produced by ligation of the left anterior descending coronary artery. Sarcoplasmic reticulum calcium content was measured by laser scanning confocal microscopy;western blot method was used to analyze the differences in expression of calcium handling proteins in sham and heart failure. Results：The fluorescence intensity of ratsmyocytesin heart failure group was much lower than the sham group, and the caffeine induced calcium transients in heart failure group was lower than that of the sham group. The expression of PLN phosphorylation level decreased, but the level of overall PLN did not change. The expression of SERCA2a decreased and the level of PP1 increased. Conclusion：The main reasons for reducing sarcoplasmic reticulum calcium uptake were the reduction of SERCA2a expression and the decrease of SERCA2a activity.
出处
《电子显微学报》
CAS
CSCD
2015年第1期55-61,共7页
Journal of Chinese Electron Microscopy Society
