Anti-DNP IgE联合DNFB诱导免疫性接触性荨麻疹小鼠模型的建立

A Mouse Model of Immunological Contact Urticaria by Anti-dinitrophenol IgE Combined with 2,4-Dinitrofluorobenzene

目的:利用抗-二硝基苯酚(anti-DNP)Ig E单克隆抗体和2,4-二硝基氟苯(DNFB)建立免疫性接触性荨麻疹小鼠模型。方法:将36只BALB/c小鼠随机分成3组,每组12只:造模组小鼠尾静脉注射anti-DNP Ig E单克隆抗体,24 h后在小鼠双耳涂DNFB进行激发;单纯Ig E组仅注射anti-DNP Ig E单克隆抗体,空白对照组小鼠注射生理盐水,均在24 h后在小鼠双耳涂生理盐水。观察小鼠在14 d内的风团、瘙痒、耳朵肿胀度情况,并检测血清中Ig E、IL-4、IFN-γ、组胺水平和皮肤病理。结果:造模组全部小鼠产生风团;与其它两组相比,造模组小鼠搔抓次数、耳朵肿胀度及组胺血清水平均明显升高(P值均<0.01),但三组间Ig E、IL-4、IFN-γ水平无明显差异(P>0.05)。结论:静脉注射anti-DNP Ig E单克隆抗体联合外用DNFB可快速、可靠地建立免疫性接触性小鼠荨麻疹模型。

Objective： To establish an animal model of immunological contact urticaria of mice.Methods： Thirty-six BALB / c mice were randomly divided into 3 groups. The mice of model group were injected with anti-dinitrophenol IgE monoclonal antibody by tail vein. The mice of control groups were injected with anti-dinitrophenol IgE monoclonal antibody or normal saline by tail vein.After 24 hours,2,4- dinitrofluorobenzene（ DNFB） was painted on the ears of model group and physiological saline was painted on the ears of control groups respectively. Mice were observed within 14 days of the wheal,pruritus,ear thickness,serum levels of IgE,IL-4 and IFN-γ,histamine and skin pathology. Results： Wheals were seen on the mice of all of mode group. Compared with control groups,scratching number,ear thickness and serum levels of histamine of the mice of model group were increased significantly（ P〈 0. 01）. However,there were no differences in serum levels of IgE,IL-4 and IFN-γ among 3 groups（ P 〉0. 05）. Conclusion： A mouse model of immunological contact urticaria would be quickly and reliably established by induction of anti-dinitrophenol IgE monoclonal antibody and DNFB.