不同组分及低磷钾胁迫的马铃薯试管苗快繁培养基研究

Study on the Rapid Propagation of Potato Culture Mediums Under Different Components,Low Phosphorus and Potassium Stress

为培养出高品质、高效率、低成本的马铃薯脱毒试管苗,以马铃薯脱毒试管苗克新13为试验材料,以MS为基础培养基,研究不同组分的快繁培养基对马铃薯试管苗农艺性状、生物量及叶绿素含量的影响。结果表明:不添加有机物的培养基,可保证试管苗健康生长;不添加微量元素和铁盐的培养基不利于试管苗的生长。不添加有机物且大量元素中添加磷、钾素减半的低磷、钾胁迫的培养基没有对试管苗的生长产生不利影响,多数指标与在CK培养基上生长的试管苗水平较接近,而且在活叶数、有效茎节数、生物量、平均根长以及平均根条数方面优于全量添加的CK培养基。从节约成本的角度考虑,不添加有机物且大量元素中磷素和钾素减半的MS培养基可以应用于马铃薯试管苗的生产中。

In order to explore the high quality,high efficiency and low cost potato detoxified tube seedling,taking potato virus-free plantlets Kexin 13 as material and taking MS as basic medium,the effect of rapid propagation culture medium with different composition on test tube seedling agronomic characters,biomass and chlorophyll content in potato were studied.The results showed that the culture medium without adding organic matter could ensure the healthy growth of plantlets in vitro;it was not conducive to the growth of plantlets in vitro without adding trace elements and ferric salt.Large number of elements added amount of nitrogen and potassium,half the number of low phosphorus,potassium stress free medium on growth of test tube seedlings had adverse effect without adding organic matter and,most indicators and in CK medium on the growth of plantlets level was close,but also in the loose leaf number and effective stem node number,biomass,average root length and average root number than the full amount of the added CK medium.Consider from the cost angle,a large number of elements in the phosphorous and potassium half MS medium could be applied to the production of potato plantlets in vitro without adding organic matter.