大豆内生菌的分离及菌核病拮抗菌的筛选

Isolation of Endophyte from Soybean and Screening of Antagonistic Strains Aganist Sclerotinia sclerotiorum

为筛选大豆菌核病菌的有效拮抗菌株,利用组织表面消毒法从大豆不同生育期的根、茎、叶和种子中分离内生菌。结果表明:共分离得到113株细菌和49株真菌。利用平板对峙培养法筛选出42株(细菌25株、真菌17株)对大豆菌核病菌有拮抗效果的内生菌株,其中18株对大豆菌核病菌菌丝生长有良好的抑制作用。

In order to screen effective antagonistic strains for Sclerotinia sclerotiorum,the endophytes were screened by way of surface sterilization from the different development stages of the soybean root,stem,leaf and seed.The results showed that 113 bacteria and 49 fungus were isolated from different tissues of soybean in its growth stage.42 antagonistic strains against Sclerotinia sclerotiorum were gotten.Among the 42 strains,18strains had been proved that they could effectively inhibit the growth of Sclerotinia sclerotiorum mycelium.