抗杜氏和什曼原虫单克隆抗体的应用——Ⅱ.内脏利什曼病治疗前后循环抗原的检测

APPLICATION OF MONOCLONAL ANTIBODIES AGAINST LEISHMANIA DONOVANI——Ⅱ. DETECTION OF CIRCULATING ANTIGEN IN SERA OF VISCERAL LEISHMANIASIS BEFORE AND AFTER TREATMENT

以抗杜庆利什曼单克隆抗体L_(12)F_7检测黑热病病人的循环抗原。文中对1986～1989年收集并低温保存的118份血清加以检测,阳性率为88.9％;另检测经锑剂治疗后的患者血清57份,结果均为阴性。同时又以IFA检测体内抗体,阳性率为68.4％。这57例治愈者经追踪观察并未出现复发及抗锑现象。因而用单克隆抗体检测循环抗原的另一优越性,可以作为疗效考核的新方法。

Monoclonal antibodies L12F7 against the target antigen of L. doreovani promastigotes were used for the detection of circulating antigen(CAg) from sera of visceral leishmaniasis pati-ents. The results showed that of 118 serum samples from visceral leishmaniasis tested. 105 were positive(88.9%). 55 normal serum samples were negative. No cross reaction with sera from patients with vivax malaria, schistosomiasis. leprosy and brucellosis, was found.The authors suggested that this assay may be used as a sensitive and new serodiagno-stic test for detecting existing infection of visceral leishmaniasis for epidemiological survey and for assessment of cure after effective treatment.