黑莓籽总多酚、总黄酮含量及其抗氧化活性

Contents of total plyphenols and total flavones and antioxidant activity of blackberry seed

采用清除DPPH自由基、ABTS自由基及铁离子还原能力法评价黑莓籽体外抗氧化活性,Folin-Ciocalteau法测其总多酚含量,Na NO2-Al Cl3比色法测定其总黄酮含量,相关系数法分析其总多酚、总黄酮与抗氧化活性间的相关性。结果显示,乙酸乙酯部位和正丁醇部位具有强的DPPH自由基[IC50=(41.93±4.05)、(64.75±5.89)μg/m L]和ABTS自由基[IC50=(2.91±0.46)、(3.18±1.01)μg/m L]清除活性,较强的铁离子还原能力[Trolox当量=(2348.73±2.78)、(1262.55±31.58)μmol/g],石油醚部位具有ABTS自由基[IC50=(21.85±0.61)μg/m L]清除活性和较弱的铁离子还原能力[Trolox当量=(123.59±10.01)μmol/g]。3个部位总多酚、总黄酮含量与清除ABTS自由基能力之间存在一定的相关性(R2分别为0.6832、0.2596);总多酚含量与还原铁离子能力(Trolox当量)之间存在一定的相关性(R2=0.990)。可见,黑莓籽具有良好的抗氧化活性。

DPPH,ABTS and ferric ion reducing power methods were used to evaluate in vitro antioxidant activity of blackberry seed. The content of polyphenols was measured using the Folin-Ciocalteau method,and the content of total flavones was measured using the Na NO2- Al Cl3 colorimetric method. Correlation between polyphenol and flavone contents and antioxidant capacity was analyzed. The results showed that ethyl acetate extract and n-butanol extract from blackberry seed had DPPH [IC50=（ 41.93 ±4.05）,（ 64.75 ±5.89） μg/m L,respectively] and ABTS radical scavenging activity[IC50=（2.91±0.46）,（3.18 ±1.01）μg/m L,respectively],and ferric ion reducing power[Trolox equivalent=（2348.73±2.78）,（1262.55±31.58）μmol/g,respectively]. Petroleum ether extract had ABTS radical scavenging activity[IC50=（21.85 ±0.61）μg/m L] and weak ferric ion reducing power[Trolox equivalent=（123.59±10.01）μmol/g]. An excellent correlation between ABTS radical scavenging activity of three extracts and the contents of total polyphenols and total flavones were observed（R2=0.6832 and0.2596）. Ferric ion reducing power was correlated with the content of total polyphenols（R2=0.990）. Blackberry seed had antioxidant activity.