鲫鱼卵唾液酸糖蛋白对免疫功能低下小鼠的免疫调节作用

Immunomodulatory effects of sialoglycoprotein in eggs of Carassius auratus on immunocompromized mice

从鲫鱼卵中提取唾液酸糖蛋白,并探讨其对免疫功能低下小鼠免疫功能的作用。Balb/c小鼠连续皮下注射氢化可的松造成免疫功能低下模型,以不同剂量的鲫鱼卵唾液酸蛋白灌胃28d后,分别测定小鼠的抗体产生水平、T细胞免疫功能、非特异性免疫功能和血清IFN-γ/IL-4。结果显示,高剂量的鲫鱼卵唾液酸糖蛋白能显著升高免疫低下小鼠的血清溶血素水平(p<0.01)和抗体细胞生成数(p<0.01),增强脾淋巴细胞增殖能力(p<0.01)和迟发型变态反应(p<0.05),显著提高腹腔巨噬细胞对鸡红细胞的吞噬率和吞噬指数(p<0.01),改善碳廓清能力(p<0.05)并升高血清中IFN-γ/IL-4水平(p<0.01)。说明鲫鱼卵唾液酸糖蛋白对免疫功能低下小鼠具有显著的免疫调节作用。

Sialoglycoprotein was extracted from eggs of Carassius auratus,and its immunomodulatory effects on immunocompromized mice were studied. Different dosage of sialoglycoprotein from eggs of Carassius auratus was given to immunodepressive model mice induced by Hydrocortisone for 28 d. The antibody level,immunological function of T cell and level of serum interferon- γ（ IFN- γ） and interleukin- 4（ IL- 4） was measured. Results showed that high-dose of sialoglycoprotein could increased the hemolysin content and numbers of antibody-forming cells（p〈0.01） and strengthened lymphocyte proliferation（p〈0.01） significantly. It could aslo increase toe incrassation（p〈0.01） and promote phagocytosis ability of celiac macrophage（p〈0.05）and serum IFN-γ/IL-4（p〈0.01） markedly. Data suggested that the sialoglycoprotein from Carassius auratus had significant immunomodulatory effects on immunocompromized mice.