雷公藤内酯醇抑制IL-5诱导的嗜酸粒细胞的存活研究

Tripholide Inhibits IL-5-Induced Eosinophil Survival

目的 ;研究白介素 - 5 (IL - 5 )对嗜酸粒细胞 (EOS)的诱导作用及雷公藤对其的抑制 ,从而探讨雷公藤治疗哮喘的作用机制。方法 :将制备的EOS加入IL - 5及不同浓度的雷公藤内酯醇共同培养 ,以观察雷公藤内酯醇对IL - 5诱导EOS存活的作用。结果 :大于 5pg/ml水平的IL - 5明显提高EOS的存活 ,且表现出一定的剂量依赖关系 ;雷公藤内酯醇抑制EOS的存活从 5 0nmol/L开始 ,10 0 0nmol/L抑制作用最强 ,而当IL - 5浓度为 10 0 0pg/ml或更高时 ,其诱导的EOS存活不能被任何浓度的雷公藤内酯醇所抑制。结论 :IL - 5能够诱导EOS的存活 ,雷公藤内酯醇能抑制IL - 5对EOS的诱导作用 。

Objective To explore the effects of tripholide on interleukine-5-induced survival of eosinophils (EOS) in vitro.Methods EOS were purified from guinea pigs by Percoll density gradient and incubated for 4 days in the presence of IL-5 with or without tripholide.Results In the absence of tripholide,IL-5enhanced EOS survival in a dose dependent manner.When EOS were incubated with varying concentration of IL-5,and completely abolished by IL-5 1000pg/ml.Conclusions Tripholide acts directly on mature EOS to decrease their viabilities,implying the tripholide may be important in the treatment of allergic and other inflammatory eosinophilic disorders.