BADH基因转入玉米自交系的研究

Study on Transferring BADH Gene into Maize Inbrid Lines

采用花粉管通道法将甜菜碱醛脱氢酶基因(BADH)转入玉米自交系京24,以提高玉米的耐盐性。在玉米植株T0代喷洒200mg/L草铵膦除草剂进行抗性试验,获得4株抗性苗。在T0代进行PCR初步检测得到3株转基因植株。在T2代通过Southern blotting以及Northern blotting检测,表明外源BADH基因已经整合并表达。对T2代株系和京24自交系(CK)在高盐胁迫下进行相对含水量、叶片电解质外泄率、甜菜碱含量、脯氨酸含量等生理指标的测定,结果表明转基因株系的生理指标明显好于对照,说明转入BADH基因能提高玉米的耐盐性。

The betaine aldehyde dehydrogenase gene （BADH） was transformed into Jing24 by the pollen tube pathway in order to improve the salt tolerance of maize. The To maize plants seedlings were sprayed with 200mg/L glufosinate herbicide for herbicide screening, and four herbicide resistant seedlings were obtained. Three transgenic lines were identified by PCR examination in To generation. The BADH exogenous gene has been proved to be integrated into maize genome and expressed by Southern blotting and Northern blotting detection in the transgenic plants. Relative water were measured in the content, leaf electrolyte 1 transgenic lines and wild eakage rate, betaine and proline and other physiological indicators type plants. The results showed that the physiological indicators of transgenic lines were significantly better than the control ance for maize. , indicating that BADH gene could improve the sah tolerance for maize.