干扰素诱导蛋白10在结核性脑膜炎诊断中的应用

The significance of detecting interferon-inducible protein 10 for diagnosis of tuberculous meningitis

目的研究干扰素诱导蛋白10(interferon-inducible protein 10,IP-10)水平在结核性脑膜炎诊断中的作用。方法用ELISA检测32例结核性脑膜炎(结脑组)和32例非结核性脑膜炎患者(非结脑组)脑脊液中IP-10水平绘制ROC曲线(receiver operating characteristic curve)确定其诊断结核性脑膜炎的临界值,并评价其敏感性、特异性和诊断效能。结果结核性脑膜炎组IP-10含量为(1164.06±450.23)pg/ml,显著高于非结核性脑膜炎组(237.02±161.37)pg/ml(P<0.001)。IP-10诊断结核性脑膜炎的临界值为605.63pg/ml,敏感性和特异性分别为87.5%和96.9%;IP-10的诊断效能与γ-干扰素(IFN-γ)相近。结论脑脊液中IP-10水平测定有助于结核性脑膜炎的诊断。

Objective To evaluate the significance of interferon-inducible protein 10 （IP-10） level in the cerebrospinal fluid for the diagnosis of tuberculous meningitis. Methods Enzyme-linked immunosorbent assay （ELISA） was adopted to detect the level of IP-10 in the cerebrospinal fluid of 32 patients with tuberculous meningitis and 32 patients with non-tuberculous meningitis. The receiver operating characteristic （ROC） curves were used to determine the cut-off value and assess the sensitivity, specificity and diagnostic efficiency. Results The concentration of IP-10 was significantly higher in tuberculous meningitis （1164.06±450.23pg/ml） than in non-tuberculous meningitis （237.02±161.37） （P〈 0. 001）. When 605.63pg/ml was used as cut-off value, the sensitivity and specificity for IP-10 in diagnosis of tuberculous meningitis were 87.5 % and 96.9%, respectively. And the diagnostic efficiency of IP-10 was similar to that of IFN-γ. Conclusion Detection of IP-10 in the cerebrospinal fluid is helpful in the diagnosis of tuberculous meningitis.