生物信息学方法预测高分子蛋白质twitchin的一级结构

Prediction of primary structure of high molecular protein twitchin by bioinformatics

目的预测高分子蛋白质twitchin基因的exon-intron结构,并确定其一级结构及结构域。方法利用生物信息学方法,从霸王莲花青螺(Lottia gigantea)基因组数据中探索有无twitchin的基因,预测twitchin的基因exon-intron结构及twitchin m RNA组成,从而确定该蛋白的一级结构,并检测其结构域;与同源蛋白进行序列比对,绘制系统树,了解其在系统进化中的位置。结果在霸王莲花青螺基因组数据中存在twitchin的基因,且该蛋白基因在基因组中横跨220 000 bp的序列区域(「sca_38」的149 839-367 346区域),预测编码twitchin的m RNA约由13 000 bp组成,推算相对分子质量约为480 000,该蛋白由Ig、Fn、kinase结构域和unique序列组成,与贻贝twitchin相比较,他们之间具有较高的同源性。结论在霸王莲花青螺中存在twitchin的基因,预测和确定了该蛋白质的一级结构。

Objective To predict the exon-intron structure of high molecular protein twitchin and determine its primary structure and domain. Methods Bioinformatics was used to explore the presence or absence of twitchin gene in the genome of Lottia gigantean and predict the exon-intron structure of the gene. The primary structure and domains of twitchin protein were identified by the prediction of m RNA. Based on the sequence alignment with homologous proteins,the position in phylogenetic tree was determined. Results The coding sequence of twitchin protein was located at a220 000 bp region（sites 149 839-367 346 of 「sca38」）in the genome of L. gigantean. The predicted m RNA of twitchin protein consisted of 13 000 bp, while the deduced relative molecular mass was about 480 000. The twitchin protein consisted of Ig, Fn, kinase domains and unique sequences, which shared high homology with that from mussel.Conclusion The twitchin existed in L. gigantean, and the primary structure of twitchin protein was predicted and determined.