银杏叶提取物对阻塞性睡眠呼吸暂停综合征合并高血压大鼠血压的影响

Effect of Ginkgo biloba extract in blood pressure of obstructive sleep apnea syndrome with hypertension in rats

目的研究银杏叶提取物(GBE)对阻塞性睡眠呼吸暂停综合征(OSAS)合并高血压大鼠血压的影响,及GBE可能的作用机制。方法 30只健康雄性SD大鼠随机均分为正常对照(NC)组、慢性间歇性低氧(CIH)组、GBE灌胃组,各组10只。于实验前后采用无创血压分析仪观察大鼠血压变化;HE染色观察大鼠心肌、肾、腹主动脉的病理变化;同时检测大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素1(IL-1)、白细胞介素6(IL-6)的含量;及外周脂肪组织中Toll样受体4(TLR4)、c-Jun氨基末端激酶(JNK)蛋白及其mRNA表达水平。结果 CIH组大鼠血压明显高于对照组(P<0.01),GBE灌胃组大鼠血压显著低于CIH组(P<0.01);与NC组相比,CIH组心肌细胞、肾小管明显水肿呈水样变性,GBE灌胃组与CIH组比较心肌细胞、肾小管水肿显著降低,各组腹主动脉均无明显病理改变;与NC组比较,CIH组大鼠血清中TNF-α、IL-1、IL-6的含量均显著增高(P<0.01),GBE灌胃组与CIH组比较大鼠血清中TNF-α、IL-1、IL-6的含量显著降低(P<0.05),GBE灌胃组与NC组比较TNF-α、IL-6的含量差异无统计学意义,而IL-1的含量GBE灌胃组明显高于NC组(P<0.01);与NC组比较,CIH组外周脂肪组织中TLR4、JNK蛋白及其mRNA表达水平显著增高(P<0.01),而GBE灌胃组其表达含量显著低于CIH组(P<0.01)。结论 GBE可以抑制OSAS合并高血压大鼠脂肪组织TLR4、JNK蛋白及mRNA的表达,降低血清中TNF-α、IL-1、IL-6的水平,对OSAS合并高血压大鼠血压有显著降压作用。

Objective To study the effect of Ginkgo biloba extract（ GBE） in blood pressure of rat obstructive sleep apnea syndrome（ OSAS） with hypertension, and the mechanism of GBE. Methods 30 healthy male rats were ran-domly divided into normal control group （NC）,chronic intermittent hypoxia group（CIH）,and GBE gastric perfu-sion group （n=10）. The changes of blood pressure, myocardial, renal and abdominal artery were observed by non-invasive blood pressure analyzer and HE staining. The levels of tumor necrosis factor-α（TNF-α）,interleukin-1（IL-1）and interleukin-6（IL-6）were examined in serum of these rats. The levels of Toll-like receptor 4（TLR4）, c-Jun N-terminal kin（ JNK） and the expression of JNK mRNA were tested in the peripheral adipose tissue. Results The blood pressure in CIH was significantly higher than that in NC（P 〈0. 01）, and blood pressure in GBE was lower than that in CIH （ P 〈0. 01 ） . Compared with NC, the pathological variation of myocardial cell and renal tubule showed significant swell in CHI, but in GBE these changes were slight than that in CHI. There were no obvious pathological changes of abdominal artery in each group. The levels of TNF-α,IL-1 and IL-6 in CHI were signifi-cantly higher than those in NC（P 〈0. 01）,and the content was lower in GBE compared with CHI（P 〈0. 05）. But the results about TNF-α,IL-6 in GBE had no statistical significance compared with NC,and the content of IL-1βwas higher than that in NC（P 〈0. 01）. The expression of TLR4,JNK and expression of JNK mRNA in CHI were signif-icantly higher than those in NC in peripheral adipose tissues（ P 〈0. 01 ） , and the results in GBE were lower than that in CHI（P 〈0. 01）. Conclusion GBE can restrain the expression of TLR4,JNK protein and JNKmRNA in the adipose tissue of rat in OSAS with hypertension. It can reduce the level of TNF-α,IL-1,IL-6 in serum. GBE has a significant antihypertensive effect on OSAS with hypertension in rats.