Peroxiredoxin 1与EMT相关蛋白在胰腺癌组织中的表达及其临床意义

Clinical significance of expression of Peroxiredoxin 1 and EMT related factors in pancreatic carcinoma

目的探讨过氧化物还原酶1(Prx1)与上皮细胞间质转化(EMT)相关蛋白在胰腺癌组织中的表达及其临床意义。方法采用免疫组化法分别检测Prx1、E-钙黏素(Ecadherin)、波形蛋白(Vimentin)在66例胰腺癌组织和11例正常胰腺组织中的表达,并分析三者之间的关系及与临床病理因素间的关系。结果 Prx1、E-cadherin和Vimentin分别在66例胰腺癌组织中的阳性表达率为69.70%(46/66)、40.91%(27/66)、56.06%(37/66);Prx1、E-cadherin和Vimentin分别在11例正常胰腺组织中的阳性表达率为18.18%(2/11)、90.90%(10/11)、18.18%(2/11)。Prx1、E-cadherin和Vimentin在胰腺癌组织中的表达均与TNM分期、淋巴结转移和肿瘤分化程度显著相关(P<0.05),而与性别、年龄、肿瘤大小、病变部位均无关;Prx1与神经侵犯显著相关(P<0.05),而E-cadherin和Vimentin与神经侵犯无关。Prx1的表达与Vimentin表达呈正相关(P<0.05),Prx1和Vimentin的表达均与E-cadherin表达呈负相关(P<0.05)。结论Prx1与EMT相关蛋白在胰腺癌组织中的表达呈显著相关性,并与其侵袭转移能力相关,提示Prx1的高表达可能为预测胰腺癌患者预后不良的辅助指标之一。

Objective To investigate the expression clinical significance of Peroxiredoxin 1 （Prxl） and epithelial mesenchymal transition （EMT） -related factors in pancreatic carcinoma. Methods The expression of Prxl, E-cad- herind and Vimentin were detected in 66 pancreatic carcinoma samples and 11 normal pancreatic tissues by immu- nohistochemical method. The correlation between Prxl, E-cadherin, Vimentin and clinicopathologic characteristics was also analyzed. Results The positive expression rates of Prxl, E-cadherind and Vimentin were 69.70% （46/ 66）, 40. 91% （27/66） and 56.06% （37/66） in pancreatic carcinoma, and 18.18% （2/11）, 90.90% （ 10/ 11 ） and 18. 18% （2/11） in normal pancreatic tissue, respectively. The expression rates of Prxl, E-cadherin and Vimentin were correlated with TNM stage, lymph node metastasis, degree of differentiation （ P 〈 0. 05 ）. Prxl expression was correlated with nerve invasion in pancreatic carcinoma tissue （P 〈 0. 05 ）. The expression rates of Prxl, E-cadherin and Vimentin were not correlated with sex, age, size, site of tumor. E-cadherin and ~imentin expression were not correlated with nerve invasion inpancreatic carcinoma tissue. Prxl expression in pancreatic car- cinoma was positively correlated with Vimentin expression （P 〈 0. 05 ） ; Prxl and Vimentin were negatively correla- ted with E-cadherin expression （P 〈 0. 05）. Conclusion Prxl, E-cadherin and Vimentin expressions are associ- ated with the ability of invasion and metastasis in pancreatic carcinoma, Prxl abnormal expression maybe serve as a potential poor prognostic marker of patients with pancreatic carcinoma.