摘要
目的探讨单采血小板的储存时间与血小板膜蛋白CD62P表达之间的相关性。方法招募自愿献血的健康献血者30名,按照血站标准采集30袋单采血小板,每袋200mL,血小板计数>2.5×1011/袋,置于22℃血小板保存箱保存7d,分别在保存的第1d、第3d、第5d、第7d通过流式细胞仪检测血小板膜蛋白CD62P。结果随着保存时间的延长,CD62P表达明显增强(P<0.05),两者呈现正相关关系。结论随着保存时间的延长,血小板体外活化程度增高。
Objective To explore the relationship between storage time and CD62P of apheresis platelets. Methods Thirty bags of apheresis platelets were collected from voluntary blood donor by blood station standard. The volume was 200mL and PLT〉2. 5 × 10 11 in each bag. All of apheresis platelets were stored in storage tank for 7 days at 22℃. CD62P was detected by flow cytometry at d1,d3,d5,d7. Results expression of CD62P was gradually increased with storage time prolonged（P〈0. 05）. Conclusion Platelet activation was gradually increased in vitro ith storage time prolonged.
出处
《四川医学》
CAS
2015年第3期418-420,共3页
Sichuan Medical Journal
关键词
单采血小板
储存时间
相关性
血小板膜蛋白
CD62P
apheresis platelets
storage time
CD62P
relationship
platelet membrane protein
