卷丹百合体外对肺腺癌A_(549)细胞抑制作用的实验研究

Depressant effects of Lilium lancifolium on human pulmonary adenocarcinoma cell line A_(549) in vitro

目的：研究百合对肺腺癌细胞株A549增殖作用的影响及其相关机制。方法：体外培养肺腺癌细胞株A549,用不同浓度的百合水提物分别作用于对数期生长的A549细胞24、48和72h,MTT（甲基噻唑基四唑）比色法检测百合对A549细胞增殖的抑制率,碘化丙啶（PI）染色,荧光显微镜下观察细胞形态改变及凋亡情况。结果：MTT法检测结果显示,百合水提物在1~8mg/mL浓度范围内、24~72h时间范围内对A549的生长有明显抑制作用,且呈浓度和时间依赖关系（P〈0.05）;PI染色荧光显微镜观察显示百合作用48h后,细胞出现凋亡的形态学改变。结论：百合具有体外抑制肺腺癌细胞株A549增殖的作用,其可能是通过诱导肿瘤细胞凋亡而发挥抑制A549细胞增殖的作用。

Objective：Investigate the effects of Lilium lancifoliumon inhibition of pulmonary adenocarcinoma cell line A549 in vitro and explore its mechanism.Methods：The A549 cells in vitro were treated with Lilium lancifolium at different concentrations to 24 h,48hand 72 hrespectively.MTT assay was used to examine the proliferation.PI staining were used to observe the morphological changes of cancer cells.Results：The depressant effects of Lilium lancifolium on human A549 were determined by MTT assay after at 24 h,48hand 72 h.The concentration of Lilium lancif olium was 1~8mg/mL.Lilium lancifolium could obviously inhibit proliferation of A549 cells,the effect was a time-dose-dependen tmanner（P〈0.05）.Pl Fluorescence microscope indicated that apoptotic change was observed in A549 cells after treatment with Lilium lancifolium for 48 h.Conclusion：Lilium lancifolium can inhibit the growth of A549 cells remarkably by inducing apoptosis and blocking cell cycle.