肾康注射液对庆大霉素肾损伤的保护作用

Protective Effect of Shenkang Injections in Rats with Kidney Injury induced by Gentamicin

目的:观察肾康注射液对庆大霉素肾损伤的保护作用。方法:48只雄性SD大鼠随机分为对照组、模型组和治疗组。模型组和治疗组大鼠腹腔注射庆大霉素(100 mg·kg-1·d-1)、持续7 d,治疗组同时予以腹腔注射肾康注射液(2 g·kg-1·d-1)治疗7 d。三组均于第8天和第15天分别处死大鼠检测血清肌酐(Cr)、胱抑素C(Cys C)以及尿液肾损伤分子(Kim)-1、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)浓度,肾组织细胞凋亡情况、核因子(NF)-κβp65活性、Bax mRNA和Bcl-2 mRNA表达以及Caspase-3活性。结果:与对照组相比,模型组第8天和第15天血清Cr和Cys C以及尿液Kim-1、NGAL浓度均升高,肾组织凋亡细胞增多,NF-κβp65活性和Caspase-3活性增强、Bax mRNA和Bcl-2 mRNA表达上调(P均<0.01);而治疗组第8d和第15d血清Cr和Cys C以及尿液Kim-1、NGAL浓度均较模型组降低,肾组织凋亡细胞减少,NF-κβp65活性和Caspase-3活性减弱、Bax mRNA表达下调、Bcl-2 mRNA表达上调,但这些指标与对照组相比均升高(P均<0.01)。结论:肾康注射液通过抑制细胞凋亡改善庆大霉素肾损伤。

Objective： To observe the protective effect of Shenkang injections in the rats with kidney injury induced by gentamicin.Methods： Forty-eight male SD rats were randomly divided into the control group,model group and treatment group with 16 ones in each. The rats were given gentamicin with intraperitoneal injection（ 100 mg·kg^-1·d^-1） for 7 days in the model group and the treatment group,and the rats in the treatment group were given Shenkang injections with intraperitoneal injection additionally（ 2. 0 g·kg^-1·d^-1）. Eight rats were sacrificed respectively on the 8th and 15 th day in each group,and the detection of serum level of creatinine and cystatin-C,urinary concentration of kidney injury molecule-1（ Kim-1） and neutrophil gelatinase-associated lipocalin（ NGAL）,activity of nuclear factor-κβ and Caspase-3,and expression of Bax mRNA and Bcl-2 mRNA in renal tissues as well as the observation of renal tissue apoptosis in each group were conducted. Results： Compared with those in the control group,the serum level of creatinine and cystatin-C,urinary concentration of Kim-1 and NGAL,renal tissue apoptosis,activity of nuclear factor-κβ and Caspase-3,expression of Bax mRNA and Bcl-2 mRNA in renal tissues on both 8th and 15 th day were higher in the model group（ P〈0. 01）. The serum level of creatinine and cystatin-C,urinary concentration of Kim-1 and NGAL,renal tissue apoptosis,activity of s nuclear factor-κβ and Caspase-3,expression of Bax mRNA in renal tissues in the treatment group were lower at the two time points than those in the model group,while the expression of Bcl-2 mRNA in homogenate renal tissues were higher in the treatment group than that in the model group at the two time points（ P〈0. 01）. All indices were higher at two time points in the treatment than those in the control group. Conclusion：Shenkang injections exhibit protective effect in rats with kidney injury induced by gentamicin through inhibiting renal tissue apoptosis.